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178613 Anti-Aquaporin 3 (275-292) Rabbit pAb

178613
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
R Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
178613-1ST Ampolla de plást. 1 st
Description
OverviewRecognizes the ~40 kDa and ~29 kDa forms of aquaporin-3 in rat kidney membrane. Supplied with a control peptide (immunogen).
Catalogue Number178613
Brand Family Calbiochem®
SynonymsAnti-AQP3, Anti-GLIP
Application Data
Detection of rat aquaporin 3 by immunoblotting. Sample: Extract from rat kidney membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (1:200). Detection: chemiluminescence.

Detection of rat aquaporin 3 by immunohistochemistry. Samples: Longitudinal section of rat colon showing mucosa (M), submucosa (SM), and muscularis externa (ME). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (Dilution Assay Dependent). Detection: fluorescence.
References
ReferencesEchevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997.
Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
Product Information
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 1% BSA, 5% sucrose, pH. 7.4 and 40 µg AQP3275-292 control peptide lyophilized from PBS.
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunohistochemistry Ishibashi, K., et al. 1997. Am. J. Physiol. 272, F235. Ecelbarger, C.A., et al. 1995. Am. J. Physiol. 269, F663.
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesImmunoblotting (1:200)
Immunohistochemistry (assay dependent; see application references)
Application CommentsFor use as a negative control, preincubate 1 µg of peptide with 1 µg of antibody for 1 h at room temperature. The epitope is unique for AQP3 and is not present in other known related proteins. It is highly homologous in mouse (17/18 identical) and human (15/18 identical) AQP3 protein. Recognition of AQP3 can be fully inhibited with the soluble AQP3275-292 blocking peptide. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [(KY)STEAENVKLAH(Nle)KHKEQI] corresponding to amino acids 275-292 of rat AQP3, with norleucine replacing methionine, conjugated to KLH
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the antibody with 50 µl deionized water to make a 300 µg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Reconstitue the peptide with 100 µl deionized water, aliquot, and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Aquaporin 3 (275-292) Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Aquaporin 3 (275-292) Rabbit pAb Certificados de análisis

CargoNúmero de lote
178613

Referencias bibliográficas

Visión general referencias
Echevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997.
Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-November-2009 RFH
SynonymsAnti-AQP3, Anti-GLIP
ApplicationImmunoblotting (1:200)
Immunohistochemistry (assay dependent; see application references)
Application Data
Detection of rat aquaporin 3 by immunoblotting. Sample: Extract from rat kidney membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (1:200). Detection: chemiluminescence.

Detection of rat aquaporin 3 by immunohistochemistry. Samples: Longitudinal section of rat colon showing mucosa (M), submucosa (SM), and muscularis externa (ME). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (Dilution Assay Dependent). Detection: fluorescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40 kDa and ~29 kDa aquaporin-3 proteins. Supplied with a control peptide (immunogen).
BackgroundAQP3 may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It is also slightly permeable to urea and glycerol. AQP3 may play an important role in gastrointestinal tract water transport and in glycerol metabolism. AQP3 has a wide tissue distribution. In the kidney it is predominantly expressed in the inner medulla.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide [(KY)STEAENVKLAH(Nle)KHKEQI] corresponding to amino acids 275-292 of rat AQP3, with norleucine replacing methionine, conjugated to KLH
IsotypeIgG
Speciesrat
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 1% BSA, 5% sucrose, pH. 7.4 and 40 µg AQP3275-292 control peptide lyophilized from PBS.
Preservative≤0.1% sodium azide
CommentsFor use as a negative control, preincubate 1 µg of peptide with 1 µg of antibody for 1 h at room temperature. The epitope is unique for AQP3 and is not present in other known related proteins. It is highly homologous in mouse (17/18 identical) and human (15/18 identical) AQP3 protein. Recognition of AQP3 can be fully inhibited with the soluble AQP3275-292 blocking peptide. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the antibody with 50 µl deionized water to make a 300 µg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Reconstitue the peptide with 100 µl deionized water, aliquot, and freeze (-20°C).
Toxicity Standard Handling
ReferencesEchevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997.
Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
Application referencesImmunohistochemistry Ishibashi, K., et al. 1997. Am. J. Physiol. 272, F235. Ecelbarger, C.A., et al. 1995. Am. J. Physiol. 269, F663.