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217595 Anti-CD40L Mouse mAb (24-31)

217595
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
217595-100UG Ampolla de plást. 100 μg
Description
OverviewRecognizes the ~36 kDa CD40L (gp39) protein on activated CD3+ human PBL.
Note: 1 T = 1 test.
Catalogue Number217595
Brand Family Calbiochem®
SynonymsAnti-gp39
References
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preservative0.5 mg/ml gentamycin sulfate
Applications
Application ReferencesImmunoblotting Oledzka, G., et al. 2007. Mol. Immunol. 44, 741.
Key Applications Immunoprecipitation
Neutralization Studies
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Application NotesFlow Cytometry (10 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
Immunoblotting (see application references)
Application CommentsThis antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x105 ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman sgp39 fusion protein
ImmunogenHuman
Clone24-31
HostMouse
Purity≥95% by SDS-PAGE
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CD40L Mouse mAb (24-31) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-CD40L Mouse mAb (24-31) Certificados de análisis

CargoNúmero de lote
217595
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
SynonymsAnti-gp39
ApplicationFlow Cytometry (10 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
Immunoblotting (see application references)
DescriptionProtein A affinity purified mouse monoclonal antibody. Recognizes the ~39-40 kDa CD40L protein.
BackgroundHuman CD40 ligand (CD40L) is a member of the tumor necrosis factor (TNF) family expressed on the surface of activated T-cells.
HostMouse
Immunogen speciesHuman
Immunogenhuman sgp39 fusion protein
Clone24-31
IsotypeIgG₁
Specieshuman
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Preservative0.5 mg/ml gentamycin sulfate
CommentsThis antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x105 ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
Application referencesImmunoblotting Oledzka, G., et al. 2007. Mol. Immunol. 44, 741.