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208880 Anti-Calnexin, C-Terminal (575-593) Rabbit pAb

208880
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
B, Ca, Gp, Ht, H, Mk, M, Rb, R, Sh Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
208880-50UL Ampolla de plást. 50 ul
Description
OverviewRecognizes the ~90 kDa calnexin protein.
Catalogue Number208880
Brand Family Calbiochem®
SynonymsAnti-IP90, Anti-p88
Application Data
Detection of human calnexin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1), Vero (lane 2), Rat-2 cells (lane 3) and L-929 cells (lane 4). Primary antibody: Anti-Calnexin, C-Terminal (575-593) Rabbit pAb (Cat. No. 208880) (1:1000). Detection: chemiluminescence.
References
ReferencesHammond, C., et al. 1994. J. Cell Biol. 126, 41.
Ou, W.-J., et al. 1993. Nature 364, 771.
David, V., et al. 1993. J. Biol. Chem. 268, 9585.
Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Positive controlHeat-shocked HeLa cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide (AEEDEILNRSPRNRKPRRE) corresponding to amino acids 575-593 of canine calnexin, conjugated to KLH
ImmunogenCanine
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Canine
  • Guinea Pig
  • Hamster
  • Human
  • Monkey
  • Mouse
  • Rabbit
  • Rat
  • Sheep
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Calnexin, C-Terminal (575-593) Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Calnexin, C-Terminal (575-593) Rabbit pAb Certificados de análisis

CargoNúmero de lote
208880

Referencias bibliográficas

Visión general referencias
Hammond, C., et al. 1994. J. Cell Biol. 126, 41.
Ou, W.-J., et al. 1993. Nature 364, 771.
David, V., et al. 1993. J. Biol. Chem. 268, 9585.
Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-January-2008 JSW
SynonymsAnti-IP90, Anti-p88
ApplicationImmunoblotting (1 µg/ml)
Application Data
Detection of human calnexin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1), Vero (lane 2), Rat-2 cells (lane 3) and L-929 cells (lane 4). Primary antibody: Anti-Calnexin, C-Terminal (575-593) Rabbit pAb (Cat. No. 208880) (1:1000). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~90 kDa calnexin protein.
BackgroundCalnexin is an ER transmembrane protein that transiently associates with newly synthesized, incompletely folded monomeric glycoproteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRµ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.
HostRabbit
Immunogen speciesCanine
Immunogena synthetic peptide (AEEDEILNRSPRNRKPRRE) corresponding to amino acids 575-593 of canine calnexin, conjugated to KLH
IsotypeIgG
Speciesbovine, canine, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep
Positive controlHeat-shocked HeLa cells
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHammond, C., et al. 1994. J. Cell Biol. 126, 41.
Ou, W.-J., et al. 1993. Nature 364, 771.
David, V., et al. 1993. J. Biol. Chem. 268, 9585.
Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.