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324390 Anti-Dopamine D₁ Receptor Rabbit pAb

324390
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
324390-100UL Ampolla de plást. 100 ul
Description
OverviewRecognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
Catalogue Number324390
Brand Family Calbiochem®
References
ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat brain
PreservativeNone
Applications
Application ReferencesImmunoblotting and Immunohistochemistry Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325. Species Reactivity Kipnis, J., et al. 2004. Journal of Neuroscience 24 6133.
Key Applications Immunoblotting (Western Blotting)
Not Paraffin Sections
Enzyme-Linked Immunosorbent Assay
Free-floating Sections
Frozen Sections
Application NotesELISA (see comments)
Immunoblotting (1:1000)
Frozen Sections (1:5000)
Free-Floating Sections (1:5000)
Paraffin Sections (not recommended)
Application CommentsThis antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Dopamine D₁ Receptor Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Dopamine D₁ Receptor Rabbit pAb Certificados de análisis

CargoNúmero de lote
324390

Referencias bibliográficas

Visión general referencias
Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.

Citas

Título
  • Jonathan Kipnis, et al. (2004) Dopamine, through the Extracellular Signal-Related Kinase Pathway, Downregulates CD4+CD25+ Regulatory T-Cell Activity: Implications for Neurodegeneration. Journal of Neuroscience 24, 6133-6143.
  • Ficha técnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision27-July-2007 RFH
    ApplicationELISA (see comments)
    Immunoblotting (1:1000)
    Frozen Sections (1:5000)
    Free-Floating Sections (1:5000)
    Paraffin Sections (not recommended)
    DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1 receptor protein.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
    IsotypeIgG
    Specieshuman, mouse, rat
    Positive controlRat brain
    FormLyophilized
    FormulationUndiluted serum.
    PreservativeNone
    CommentsThis antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsReconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
    Toxicity Standard Handling
    ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
    Citation
  • Jonathan Kipnis, et al. (2004) Dopamine, through the Extracellular Signal-Related Kinase Pathway, Downregulates CD4+CD25+ Regulatory T-Cell Activity: Implications for Neurodegeneration. Journal of Neuroscience 24, 6133-6143.
  • Application referencesImmunoblotting and Immunohistochemistry Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325. Species Reactivity Kipnis, J., et al. 2004. Journal of Neuroscience 24 6133.