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324393 Anti-Dopamine D₂ Receptor Rabbit pAb

324393
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H, R Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
324393-100UL Ampolla de plást. 100 ul
Description
OverviewRecognizes the dopamine D2 receptor in rat brain. Does not cross-react with other dopamine receptors and exhibits minimal cross-reactivity with the D2S short receptor.
Catalogue Number324393
Brand Family Calbiochem®
References
ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat brain
PreservativeNone
Applications
Application ReferencesFrozen Sections Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesELISA (see comments)
Immunoblotting (1:800)
Frozen Sections (1:5000, see application references)
Application CommentsThis antibody is highly specific for the immunizing peptide sequence and is suitable for detection of both the D2S and D2L dopamine receptors in human and rat samples. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted antiserum detects two bands at ~48 and ~51 kDa in an analysis using whole rat brain homogenates. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections, a 1:5000 dilution of the reconstituted antiserum demonstrates specific staining of rat brain sections including the plasma membrane in the cortex, caudate putamen, globus pallidus, thalamic nucleus, supra optic nucleus, and the dentate gyrus. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (CAARRAQELEME) corresponding to amino acids 272-282 of human D₂ dopamine receptor
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Dopamine D₂ Receptor Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Dopamine D₂ Receptor Rabbit pAb Certificados de análisis

CargoNúmero de lote
324393

Referencias bibliográficas

Visión general referencias
Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-July-2007 RFH
ApplicationELISA (see comments)
Immunoblotting (1:800)
Frozen Sections (1:5000, see application references)
DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa dopamine D2 receptor protein.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (CAARRAQELEME) corresponding to amino acids 272-282 of human D₂ dopamine receptor
IsotypeIgG
Specieshuman, rat
Positive controlRat brain
FormLyophilized
FormulationUndiluted serum.
PreservativeNone
CommentsThis antibody is highly specific for the immunizing peptide sequence and is suitable for detection of both the D2S and D2L dopamine receptors in human and rat samples. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted antiserum detects two bands at ~48 and ~51 kDa in an analysis using whole rat brain homogenates. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections, a 1:5000 dilution of the reconstituted antiserum demonstrates specific staining of rat brain sections including the plasma membrane in the cortex, caudate putamen, globus pallidus, thalamic nucleus, supra optic nucleus, and the dentate gyrus. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Toxicity Standard Handling
ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Application referencesFrozen Sections Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.