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324405 Anti-Dopamine D₄ Receptor Rabbit pAb

324405
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H, R Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
324405-100UL Ampolla de plást. 100 ul
Description
OverviewRecognizes the ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors.
Catalogue Number324405
Brand Family Calbiochem®
References
ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat brain
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesELISA (see comments)
Immunoblotting (1:500)
Immunohistochemistry (1:10,000)
Application CommentsThis antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
Biological Information
Immunogena synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Dopamine D₄ Receptor Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Dopamine D₄ Receptor Rabbit pAb Certificados de análisis

CargoNúmero de lote
324405

Referencias bibliográficas

Visión general referencias
Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-July-2007 RFH
ApplicationELISA (see comments)
Immunoblotting (1:500)
Immunohistochemistry (1:10,000)
DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
IsotypeIgG
Specieshuman, rat
Positive controlRat brain
FormLyophilized
FormulationUndiluted serum.
PreservativeNone
CommentsThis antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
Toxicity Standard Handling
ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.