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341286 Anti-Farnesyl Rabbit pAb

341286
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
A Broad Range Of Species Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
341286-100UG Ampolla de plást. 100 μg
Description
OverviewRecognizes farnesyl. Also recognizes KLH.
Catalogue Number341286
Brand Family Calbiochem®
Application Data
Detection of farnesyl by immunofluorescence. Sample: Plasmids encoding isoprenylated protein. Primary antibody: Anti-Farnesyl Rabbit pAb (Cat. No. 341286) (assay dependent on Primary Antibody). Detection: fluorescence.
References
ReferencesBaron, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11626.
Lin, H.P., et al. 1999. J. Gen. Virol. 80, 91.
Aspbury, R.A., et al. 1998. Biochim Biophys Acta 1392, 265.
Gromov, P.S., et al. 1996. Electrophoresis 17, 1728.
Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem. 65, 241.
Product Information
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunofluorescence
Application NotesELISA (1:1000)
Immunofluorescence (1:50)
Immunoblotting (not recommended; see comments)
Application CommentsRecognizes proteins that have been isoprenylated by farnesyltransferase of geranylgeranyltransferase at the C-A-A-X motif. Also recognizes KLH. Not suitable for immunoblotting due to the hydrophobicity of the isoprenylated motif. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogenfarnesyl cysteine conjugated to KLH
HostRabbit
IsotypeIgG
Species Reactivity
  • A Broad Range Of Species
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Farnesyl Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Farnesyl Rabbit pAb Certificados de análisis

CargoNúmero de lote
341286

Referencias bibliográficas

Visión general referencias
Baron, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11626.
Lin, H.P., et al. 1999. J. Gen. Virol. 80, 91.
Aspbury, R.A., et al. 1998. Biochim Biophys Acta 1392, 265.
Gromov, P.S., et al. 1996. Electrophoresis 17, 1728.
Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem. 65, 241.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-October-2007 RFH
ApplicationELISA (1:1000)
Immunofluorescence (1:50)
Immunoblotting (not recommended; see comments)
Application Data
Detection of farnesyl by immunofluorescence. Sample: Plasmids encoding isoprenylated protein. Primary antibody: Anti-Farnesyl Rabbit pAb (Cat. No. 341286) (assay dependent on Primary Antibody). Detection: fluorescence.
DescriptionPurified rabbit polyclonal antibody. Recognizes farnesyl.
BackgroundPrenylation of proteins is a post-translational modification that is necessary for membrane association of proteins as well as protein-protein interactions. The nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors. Approximately 0.5% of cellular proteins are affected by protein isoprenylation. Farnesyltransferase and geranylgeranyltransferase are the two enzymes involved in catalyzing the attachment of two prenyl groups to the sulfhydryl group of the C-terminal cysteine groups. These enzymes transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C= Cysteine, A1 & A2 = aliphatic amino acids). Some of the proteins that contain the C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins, and the γ-subunit of trimeric G proteins.
HostRabbit
Immunogenfarnesyl cysteine conjugated to KLH
IsotypeIgG
Speciesa broad range of species
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsRecognizes proteins that have been isoprenylated by farnesyltransferase of geranylgeranyltransferase at the C-A-A-X motif. Also recognizes KLH. Not suitable for immunoblotting due to the hydrophobicity of the isoprenylated motif. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBaron, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11626.
Lin, H.P., et al. 1999. J. Gen. Virol. 80, 91.
Aspbury, R.A., et al. 1998. Biochim Biophys Acta 1392, 265.
Gromov, P.S., et al. 1996. Electrophoresis 17, 1728.
Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem. 65, 241.