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371732 Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb

371732
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
Ma Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
371732-50UL Frasco de vidrio 50 ul
Description
OverviewRecognizes both large and small forms of Gsα-subunit. Does not cross-react with Giα-1-, Giα-2-, Giα-3-, or Goα-subunits.
Catalogue Number371732
Brand Family Calbiochem®
References
ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
Product Information
FormLiquid
FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
Positive controlGsα-Subunit, His•Tag®, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or Gsα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsDoes not cross-react with Giα-1, Giα-2, Giα-3, or Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian Gsα subunit, conjugated to KLH
HostRabbit
IsotypeIgG
Species Reactivity
  • Mammals
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb Certificados de análisis

CargoNúmero de lote
371732

Referencias bibliográficas

Visión general referencias
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.

Citas

Título
  • Alexandre Charbonneau, et al. (2005) Alterations in hepatic glucagon receptor density and in Gsa and Gia2 protein content with diet-induced hepatic steatosis: effects of acute exercise. American Journal of Physiology Endocrinology and Metabolism 289, E8-E14.
  • Ficha técnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-August-2007 RFH
    ApplicationImmunoblotting (1:1000)
    DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa Gsα subunit protein.
    HostRabbit
    Immunogena synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian Gsα subunit, conjugated to KLH
    IsotypeIgG
    Speciesmammalian
    Positive controlGsα-Subunit, His•Tag®, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or Gsα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)
    FormLiquid
    FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
    PreservativeNone
    CommentsDoes not cross-react with Giα-1, Giα-2, Giα-3, or Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
    Storage ≤ -70°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling
    ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
    Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
    Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
    Citation
  • Alexandre Charbonneau, et al. (2005) Alterations in hepatic glucagon receptor density and in Gsa and Gia2 protein content with diet-induced hepatic steatosis: effects of acute exercise. American Journal of Physiology Endocrinology and Metabolism 289, E8-E14.