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196800 Bax Activator, BAM7 - Calbiochem

196800
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Empirical Formula
C₂₁H₁₉N₅O₂S • HBr

Products

Número de referenciaEmbalaje Cant./Env.
196800-10MG Frasco de vidrio 10 mg
Description
Overview

This product has been discontinued.



A cell-permeable pyrazolone compound that triggers Bax conformational changes by targeting Bax N-terminal face "BH3 trigger site" formed by the confluence of α-helices 1 &2 in a reversible manner (IC50 = 4.41 µM against 50 nM BIM BH3-derived peptide for Bax binding), leading to dose-dependent Bax oligomerization induction, Bax mitochondria translocation, and eventual Bax-dependent apoptotic cell death (33%, 90% and 94% survival rate in Bak-/-, Bak-/- Bax-/-, and Bax-/- MEF cultures, respectively; 10 µM for 24 h).

Catalogue Number196800
Brand Family Calbiochem®
SynonymsBax Activating Molecule 7, (E)-4-(2-(2-Ethoxyphenyl)hydrazono)-3-methyl-1-(4-phenylthiazol-2-yl)-1H-pyrazol-5(4H)-one, HBr
References
ReferencesGavathiotis, E., et al. 2012. Nat. Chem. Biol. 8, 639.
Product Information
FormOrange powder
Hill FormulaC₂₁H₁₉N₅O₂S • HBr
Chemical formulaC₂₁H₁₉N₅O₂S • HBr
ReversibleY
Structure formula ImageStructure formula Image
Applications
Biological Information
Primary TargetBax
Purity≥99% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Bax Activator, BAM7 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Referencias bibliográficas

Visión general referencias
Gavathiotis, E., et al. 2012. Nat. Chem. Biol. 8, 639.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-February-2013 JSW
SynonymsBax Activating Molecule 7, (E)-4-(2-(2-Ethoxyphenyl)hydrazono)-3-methyl-1-(4-phenylthiazol-2-yl)-1H-pyrazol-5(4H)-one, HBr
DescriptionA cell-permeable pyrazolone compound that triggers Bax conformational changes by targeting Bax N-terminal face "BH3 trigger site" formed by the confluence of α-helices 1 &2 in a reversible manner (IC50 = 4.41 µM against 50 nM BIM BH3-derived FITC-BIM SAHB peptide for Bax binding), leading to dose-dependent Bax oligomerization induction, Bax mitochondria translocation, and eventual Bax-dependent apoptotic cell death (33%, 90% and 94% survival rate in Bak-/-, Bak-/- Bax-/-, and Bax-/- MEF cultures, respectively; 10 µM for 24 h). BAM7 is shown to exhibit selective affinity toward Bax, but not N-terminal BIM BH3 binding site in the proapoptotic Bak, or the antiapoptotic BCL-XL, MCL-1, and Bfl-1/A1. The N-terminal "Bax trigger site" is distinct from the canonical BH3-binding site at the C-terminal face of Bcl-2 family proteins and Bax K21E mutation is shown to abolish BAM7 binding.
FormOrange powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₁H₁₉N₅O₂S • HBr
Structure formulaStructure formula
Purity≥99% by HPLC
SolubilityDMSO (5 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesGavathiotis, E., et al. 2012. Nat. Chem. Biol. 8, 639.