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203630 Bmi1-Ring1A E3 Ligase Inhibitor, PRT4165 - CAS 31083-55-3 - Calbiochem

203630
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Número de referenciaEmbalaje Cant./Env.
203630-25MG Frasco de vidrio 25 mg
Description
OverviewA cell-permeable indandione compound that inhibits the Bmi1-Ring1A E3 complex ubiquitin ligase activity both in cell-free assays (by 100% against self- and Top2α ubiquitination at 25 and 50 µM, respectively) and in HeLa cells (by 100% against Bmi1 ubiquitination at 50 µM). Shown to greatly potentiate Teniposide (VM26) cancer cytotoxicity (by 10-fold in A375 and A549 cultures at 5.5 and 33 µM, respectively) by preventing Top2α degradation following VM26 treatment (100% inhibition at 50 µM in HeLa cells).
Catalogue Number203630
Brand Family Calbiochem®
Synonyms2-(Pyridin-3-ylmethylene)-1H-indene-1,3(2H)-dione
References
ReferencesAlcganati, I., et al. 2009. PLosOne 4, e8104.
Product Information
CAS number31083-55-3
FormYellow powder
Hill FormulaC₁₅H₉NO₂
Chemical formulaC₁₅H₉NO₂
Structure formula ImageStructure formula Image
Applications
Biological Information
Primary TargetBMI1/RING1A
Purity≥99% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-207deg;C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Bmi1-Ring1A E3 Ligase Inhibitor, PRT4165 - CAS 31083-55-3 - Calbiochem Certificados de análisis

CargoNúmero de lote
203630

Referencias bibliográficas

Visión general referencias
Alcganati, I., et al. 2009. PLosOne 4, e8104.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-September-2013 JSW
Synonyms2-(Pyridin-3-ylmethylene)-1H-indene-1,3(2H)-dione
DescriptionA cell-permeable indandione compound that inhibits the activity of the E3 ubiquitin ligase complex Bmi1-Ring1A both in cell-free assays (complete inhibition of self- and Top2α ubiquitination with 25 and 50 µM PRT4165, respectively) and in HeLa cells (complete inhibition of Bmi1 ubiquitination by 50 µM PRT4165; 5 h incubation). Modifies the cellular localization of Bmi1 without affecting its expression levels. Shown to greatly potentiate Teniposide (VM26) cancer cytotoxicity (by 10-fold in A375 melanoma and A549 lung cancer cultures with 5.5 and 33 µM PRT4165, respectively) by preventing Top2α degradation following VM26 treatment (100% inhibition with 50 µM PRT4165 after 4 h 100 µM VM26 treatment in HeLa cells).
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
CAS number31083-55-3
Chemical formulaC₁₅H₉NO₂
Structure formulaStructure formula
Purity≥99% by HPLC
SolubilityDMSO (50 mg/ml). Use only fresh DMSO for reconstitution.
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-207deg;C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesAlcganati, I., et al. 2009. PLosOne 4, e8104.