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233553 COCO, Human, Recombinant

233553
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Purity
≥95% by SDS-PAGE

Products

Número de referenciaEmbalaje Cant./Env.
233553-50UG Frasco de vidrio 50 μg
Description
OverviewRecombinant, human COCO consisting of amino acids 23-189 and expressed in E. coli with an N-terminal methionine. Inhibits BMP-4 induced activity in MC3T3-E1 mouse osteoblastic cells. ED 50 = 0.15-0.75 µg/ml. Human COCO, at the amino acid sequence level, is 60% and 24% identical with mouse and Xenopus COCO, respectively.
Catalogue Number233553
Brand Family Calbiochem®
SynonymsDAND5 Dante CKTSF1B3
References
ReferencesBaron, M.H., 2005. Exp. Hematol. 33, 1015. Katoh, M. and M. Katoh. 2005. Int. J. Mol. Med. 15, 885. Kishigami, S. and Y. Mishina. 2005. Cytokine Growth FactorRev. 16, 265. AvsianKretchmer, O. and A.J.W. Hsueh. 2004. Mol. Endocrinol. 18, 1. De Robertis, E.M. and H. Koruda. 2004. Annu. Rev. Cell Dev. Biol. 20, 285. Katoh, M. and M. Katoh. 2004. Oncol. Rep. 12, 423. Bell, E., et al. 2003. Development 130, 1381. Pearce, J.J. et al., 1999. Dev. Biol. 209, 98.
Product Information
FormLiquid
FormulationLyophilized from 0.2 µm sterile-filtered Acetonitrile, TFA, BSA.
Applications
Biological Information
Biological activityED₅₀ = 0.15 0.75 µg/ml in the presence of 50 ng/ml of rhBMP-4 as measured by its ability to inhibit BMP-4-induced activity in MC3T3-E1 mouse osteoblastic cells.
Purity≥95% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

COCO, Human, Recombinant Certificados de análisis

CargoNúmero de lote
233553

Referencias bibliográficas

Visión general referencias
Baron, M.H., 2005. Exp. Hematol. 33, 1015. Katoh, M. and M. Katoh. 2005. Int. J. Mol. Med. 15, 885. Kishigami, S. and Y. Mishina. 2005. Cytokine Growth FactorRev. 16, 265. AvsianKretchmer, O. and A.J.W. Hsueh. 2004. Mol. Endocrinol. 18, 1. De Robertis, E.M. and H. Koruda. 2004. Annu. Rev. Cell Dev. Biol. 20, 285. Katoh, M. and M. Katoh. 2004. Oncol. Rep. 12, 423. Bell, E., et al. 2003. Development 130, 1381. Pearce, J.J. et al., 1999. Dev. Biol. 209, 98.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-Sepmtember-2011 RFH
SynonymsDAND5 Dante CKTSF1B3
DescriptionRecombinant, human COCO consisting of amino acids 23-189 and expressed in E. coli with an N-terminal methionine. Inhibits BMP-4 induced activity in MC3T3-E1 mouse osteoblastic cells. ED 50 = 0.15-0.75 µg/ml. Human COCO, at the amino acid sequence level, is 60% and 24% identical with mouse and Xenopus COCO, respectively.
FormLiquid
FormulationLyophilized from 0.2 µm sterile-filtered Acetonitrile, TFA, BSA.
Purity≥95% by SDS-PAGE
Biological activityED₅₀ = 0.15 0.75 µg/ml in the presence of 50 ng/ml of rhBMP-4 as measured by its ability to inhibit BMP-4-induced activity in MC3T3-E1 mouse osteoblastic cells.
SolubilityReconstitute to 200 µg/ml in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesBaron, M.H., 2005. Exp. Hematol. 33, 1015. Katoh, M. and M. Katoh. 2005. Int. J. Mol. Med. 15, 885. Kishigami, S. and Y. Mishina. 2005. Cytokine Growth FactorRev. 16, 265. AvsianKretchmer, O. and A.J.W. Hsueh. 2004. Mol. Endocrinol. 18, 1. De Robertis, E.M. and H. Koruda. 2004. Annu. Rev. Cell Dev. Biol. 20, 285. Katoh, M. and M. Katoh. 2004. Oncol. Rep. 12, 423. Bell, E., et al. 2003. Development 130, 1381. Pearce, J.J. et al., 1999. Dev. Biol. 209, 98.