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208706 Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda

208706
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
208706-5000U Ampolla de plást. 5000 u
Description
OverviewRecombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. Reportedly involved in the modulation of neurite outgrowth by cAMP. Lower levels of CaM kinase II α-subunit predispose neurons to ischemic damage.
Catalogue Number208706
Brand Family Calbiochem®
SynonymsCaM Kinase II
References
ReferencesTashima, K., et al. 1996. J. Neurochem. 66, 57.
Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Product Information
Activity≥250,000 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween® 20 Detergent, pH 7.5.
Applications
Biological Information
Purity≥90% by SDS-PAGE
Physicochemical Information
ContaminantsPhosphatases, proteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda Certificados de análisis

CargoNúmero de lote
208706

Referencias bibliográficas

Visión general referencias
Tashima, K., et al. 1996. J. Neurochem. 66, 57.
Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.

Folleto

Cargo
Calmodulin and Related Products Technical Bulletin
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-May-2008 JSW
SynonymsCaM Kinase II
DescriptionRecombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween® 20 Detergent, pH 7.5.
Recommended reaction conditions1. Dilute CaM kinase II into 20 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT, and 0.1 mM EDTA, pH 7.5.2. 2. Add 2.4 μM calmodulin, 2 mM CaCl2, 100 µM ATP, and [32P]-γATP at 100 µCi/µmole 3. Incubate at 30°C.
Purity≥90% by SDS-PAGE
ContaminantsPhosphatases, proteases: none detected
Activity≥250,000 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesTashima, K., et al. 1996. J. Neurochem. 66, 57.
Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.