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205536 Cadein1 - Calbiochem

205536
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Empirical Formula
C₃₃H₄₈F₂INO₂

Products

Número de referenciaEmbalaje Cant./Env.
205536-10MG Frasco de vidrio 10 mg
Description
OverviewA cell-permeable isoquinolinium compound that preferentially induces apoptotic cell death in p53-deficient cancer cells with functional MMR (DNA mismatch repair) system, including HCT116-Ch3/E6, HeLa, HT29, and SW620 (viability <19 %; 4-6 µM Cadein1 for 24 h), while exhibiting much reduced potency in MMR-/p53+ (RKO and HCT116), MMR-/p53- (DLD-1 and HCT116-E6), and MMR+/p53+ (HCT116-Ch3 and HEK293) cancer cultures (viability = 40 to 92%; 4 µM Cadein1 for 24 h).
Catalogue Number205536
Brand Family Calbiochem®
Synonyms1-Pentadecyl-6,7-dimethoxy-2-(2,6-difluorobenzyl)-3,4-dihydroisoquinolium chloride, Cancer-selective death inducer 1
References
ReferencesJang, E.R., et al. 2010. J. Biol. Chem. 285, 2986.
Product Information
FormYellow powder
Hill FormulaC₃₃H₄₈F₂INO₂
Chemical formulaC₃₃H₄₈F₂INO₂
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Applications
Biological Information
Purity≥97% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Cadein1 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Cadein1 - Calbiochem Certificados de análisis

CargoNúmero de lote
205536

Referencias bibliográficas

Visión general referencias
Jang, E.R., et al. 2010. J. Biol. Chem. 285, 2986.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-October-2012 JSW
Synonyms1-Pentadecyl-6,7-dimethoxy-2-(2,6-difluorobenzyl)-3,4-dihydroisoquinolium chloride, Cancer-selective death inducer 1
DescriptionA cell-permeable isoquinolinium compound that preferentially induces p38 phosphorylation, caspase-3 activation, PARP cleavage, and apoptotic cell death in p53-deficient cancer cells with functional MMR (DNA mismatch repair) system, including HCT116-Ch3/E6, HT29, SW620 (viability = 10.6, 32, and 18.6%, respectively; 4 µM Cadein1 for 24 h), and HeLa (17.7% viability; 6 µM Cadein1 for 24 h), while exhibiting much reduced potency toward MMR-/p53+ (80% and 68.6% viability of RKO and HCT116, respectively; 4 µM Cadein1 for 24 h), MMR-/p53- (51% and 49% viability of DLD-1 and HCT116-E6, respectively; 4 µM Cadein1 for 24 h), or MMR+/p53+ (55.4% and 92% viability of HCT116-Ch3 and HEK293, respectively; 4 µM Cadein1 for 24 h) cancer cells. MMR function knockdown in MMR+/p53- SW620 cells confers Cadein1 resistance (70% viability; 4 µM Cadein1 for 24 h), likewise p53 downregulation in MMR+/p53+ HEK293 cells restores sensitivity to Cadein1 (45% viability; 4 µM Cadein1 for 24 h).
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₃₃H₄₈F₂INO₂
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityDMSO (100 mg/ml)
Storage -20°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesJang, E.R., et al. 2010. J. Biol. Chem. 285, 2986.