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208772 Calpain-1 Substrate II, Fluorogenic - Calbiochem

208772
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
208772-1MG Frasco de vidrio 1 mg
Description
OverviewAn internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively.
Catalogue Number208772
Brand Family Calbiochem®
SynonymsH-E(EDANS)PLF~AERK(DABCYL)-OH
References
ReferencesCuerrier, D., et al. 2007. J. Biol. Chem. 282, 9600.
Cuerrier, D., et al. 2005. J. Biol. Chem. 280, 40632.
Product Information
ATP CompetitiveN
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₇₂H₉₇N₁₇O₁₆S
Chemical formulaC₇₂H₉₇N₁₇O₁₆S
Hygroscopic Hygroscopic
ReversibleN
Applications
Biological Information
Primary TargetCalpain-1 Substrate
Purity≥97% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceH₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Calpain-1 Substrate II, Fluorogenic - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Calpain-1 Substrate II, Fluorogenic - Calbiochem Certificados de análisis

CargoNúmero de lote
208772

Referencias bibliográficas

Visión general referencias
Cuerrier, D., et al. 2007. J. Biol. Chem. 282, 9600.
Cuerrier, D., et al. 2005. J. Biol. Chem. 280, 40632.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-January-2008 JSW
SynonymsH-E(EDANS)PLF~AERK(DABCYL)-OH
DescriptionAn internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Excitation: ~335 nm; emission: ~505 nm.
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₇₂H₉₇N₁₇O₁₆S
Peptide SequenceH₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H
Purity≥97% by HPLC
SolubilityDMSO (5 mg/ml)
Storage -20°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Irritant
ReferencesCuerrier, D., et al. 2007. J. Biol. Chem. 282, 9600.
Cuerrier, D., et al. 2005. J. Biol. Chem. 280, 40632.