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208773 Calpain Substrate IV, Cell-Permeable, Fluorogenic - Calbiochem

208773
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
208773-1MG Ampolla de plást.
Description
OverviewThe non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
Catalogue Number208773
Brand Family Calbiochem®
Synonyms(DABCYL)-TPLK~SPPPSPRE(EDANS)-RRRRRRR-NH₂
References
ReferencesBanoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.
Product Information
FormLyophilized solid
FormulationSupplied as a trifluoroacetate salt
Hill FormulaC₁₂₇H₂₀₆N₅₀O₂₇S
Chemical formulaC₁₂₇H₂₀₆N₅₀O₂₇S
Hygroscopic Hygroscopic
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Emission max.
Excitation max.
Peptide Sequence(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Calpain Substrate IV, Cell-Permeable, Fluorogenic - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Calpain Substrate IV, Cell-Permeable, Fluorogenic - Calbiochem Certificados de análisis

CargoNúmero de lote
208773

Referencias bibliográficas

Visión general referencias
Banoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-July-2014 JSW
Synonyms(DABCYL)-TPLK~SPPPSPRE(EDANS)-RRRRRRR-NH₂
DescriptionThe non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
FormLyophilized solid
FormulationSupplied as a trifluoroacetate salt
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₂₇H₂₀₆N₅₀O₂₇S
Peptide Sequence(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage -20°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesBanoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.