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218696 Casein Kinase I Inhibitor, D4476 - CAS 301836-43-1 - Calbiochem

218696
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Descripción

Replacement Information

Tabla espec. clave

Empirical FormulaCAS #
C₂₃H₁₈N₄O₃ 301836-43-1

Products

Número de referenciaEmbalaje Cant./Env.
218696-1MG Ampolla de plást. 1 mg
Description
OverviewA cell-permeable triaryl substituted imidazolo compound that acts as a potent, reversible and relatively specific ATP-competitive inhibitor of CK1 (IC50 = 0.2 µM from S. pombe; 0.3 µM for CK1δ) and ALK5 (IC50 = 0.5 µM). A weak inhibitor of p38α MAP kinase (IC50 = 12 µM) and only weakly affects the activities of a panel of kinases tested, including PKB, SGK and GSK-3β. Suppresses the site-specific phosphorylation (Ser322 and Ser325) and nuclear exclusion of FOXO1a in H4IIE hepatoma cells. Shown to be ~10-fold more potent than IC261 (Cat. No. 400090; IC50 = 2.5 µM for CK1).
Catalogue Number218696
Brand Family Calbiochem®
SynonymsCK1 Inhibitor, 4-(4-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl)benzamide
References
ReferencesRena, G., et al. 2004. EMBO Reports 5, 60.
Product Information
CAS number301836-43-1
ATP CompetitiveY
FormCrystalline solid
Hill FormulaC₂₃H₁₈N₄O₃
Chemical formulaC₂₃H₁₈N₄O₃
ReversibleY
Structure formula ImageStructure formula Image
Applications
Biological Information
Primary TargetCK1, CK1d
Primary Target IC<sub>50</sub>0.2 µM against CK1 from S. pombe; 0.3 µM for CK1δ 0.5 µM against ALK5
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Casein Kinase I Inhibitor, D4476 - CAS 301836-43-1 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Casein Kinase I Inhibitor, D4476 - CAS 301836-43-1 - Calbiochem Certificados de análisis

CargoNúmero de lote
218696

Referencias bibliográficas

Visión general referencias
Rena, G., et al. 2004. EMBO Reports 5, 60.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-November-2009 RFH
SynonymsCK1 Inhibitor, 4-(4-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl)benzamide
DescriptionA cell-permeable, reversible, potent, and relatively specific ATP-competitive inhibitor of CK1 (IC50 = 200 nM from S. pombe; 0.3 µM for CK1δ) and ALK5 (IC50 = 500 nM). A weak inhibitor of p38α MAP kinase (IC50 = 12 µM) and several other kinases tested, including PKB, SGK, and GSK-3β. Suppresses the site-specific phosphorylation (Ser322 and Ser325) and nuclear exclusion of FOXO1A in H4IIE hepatoma cells. Shown to be ~ 10-fold more potent than IC261 (Cat. No. 400090; IC50 = 2.5 µM for CK1).
FormCrystalline solid
Intert gas (Yes/No) Packaged under inert gas
Recommended reaction conditionsFor use in cell culture medium dilute 1 µl of 40 mg/ml stock solution in a mixture of 6 µl serum-free medium and 3 µl GeneJuice® Transfection Reagent (Cat. No. 70967) at +21°C; add the mixture dropwise with swirling to cultured cells.
CAS number301836-43-1
Chemical formulaC₂₃H₁₈N₄O₃
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (40 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesRena, G., et al. 2004. EMBO Reports 5, 60.