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218813 Caspase-2, Human, Recombinant, E. coli

218813
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
218813-100U Ampolla de plást. 100 u
Description
OverviewRecombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.
Catalogue Number218813
Brand Family Calbiochem®
SynonymsICH-1, Nedd-2
References
ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Wang, L., et al. 1994. Cell 78, 739.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=218820">Cat. No. 218820</a>) per h at 37°C, pH 7.2.
FormLyophilized solid
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥1500 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Caspase-2, Human, Recombinant, E. coli Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Caspase-2, Human, Recombinant, E. coli Certificados de análisis

CargoNúmero de lote
218813

Referencias bibliográficas

Visión general referencias
Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Wang, L., et al. 1994. Cell 78, 739.

Folleto

Cargo
Caspases and other Apoptosis Related Tools Brochure
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-June-2008 RFH
SynonymsICH-1, Nedd-2
DescriptionRecombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.
FormLyophilized solid
Recommended reaction conditions50 mM HEPES, pH 7.2, 50 mM NaCl, 0.1% CHAPS (Cat. No. 220201), 10 mM EDTA, 5% glycerol, 10 mM DTT, and 200 µM peptide substrate. For analyzing caspase-2 activity, use 1 unit of enzyme per assay.
Purity≥90% by SDS-PAGE
Specific activity≥1500 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-pNA (Cat. No. 218820) per h at 37°C, pH 7.2.
SolubilityPBS (0.1-1 unit/µl)
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
Toxicity Standard Handling
ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Wang, L., et al. 1994. Cell 78, 739.