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235423 Caspase-3 Inhibitor I, Cell-Permeable - Calbiochem

235423
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Empirical Formula
C₉₄H₁₅₈N₂₀O₂₇

Products

Número de referenciaEmbalaje Cant./Env.
235423-1MG Ampolla de plást. 1 mg
Description
OverviewA cell-permeable inhibitor of caspase-3, as well as caspase-6, caspase-7, caspase-8, and caspase-10. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent, and reversible inhibitor of caspase-3 (Ki < 1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC50 = 200 pM). The N-terminal sequence (amino acid residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide. A 5 mM (1 mg/100 µl) solution of Caspase-3 Inhibitor I, Cell-permeable (Cat. No. 235427) in DMSO is also available.
Catalogue Number235423
Brand Family Calbiochem®
SynonymsCPP32/Apopain Inhibitor, Cell-permeable, Cell-permeable, DEVD-CHO
References
ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Tewari, M., et al. 1995. Cell 81, 801.
Lazebnik, Y.A., et al. 1994. Nature 371, 346.
Product Information
ATP CompetitiveN
FormLyophilized solid
Hill FormulaC₉₄H₁₅₈N₂₀O₂₇
Chemical formulaC₉₄H₁₅₈N₂₀O₂₇
ReversibleY
Sold on the basis of peptide contentY
Applications
Biological Information
Primary Targetcaspase-3
Primary Target IC<sub>50</sub>200 pM for inhibiting PARP cleavage in cultured human osteosarcoma cell extracts
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Peptide ContentY
Peptide SequenceAc-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Caspase-3 Inhibitor I, Cell-Permeable - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Caspase-3 Inhibitor I, Cell-Permeable - Calbiochem Certificados de análisis

CargoNúmero de lote
235423

Referencias bibliográficas

Visión general referencias
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Tewari, M., et al. 1995. Cell 81, 801.
Lazebnik, Y.A., et al. 1994. Nature 371, 346.

Folleto

Cargo
Caspases and other Apoptosis Related Tools Brochure

Citas

Título
  • Jurg Grunenfelder, et al. (2001) Upregulation of Bcl-2 through caspase-3 inhibition ameliorates ischemia/reperfusion injury in rat cardiac allografts. Circulation 104, I202-I206.
  • Ficha técnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision06-February-2014 JSW
    SynonymsCPP32/Apopain Inhibitor, Cell-permeable, Cell-permeable, DEVD-CHO
    DescriptionA cell-permeable inhibitor of caspase-3. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent and reversible inhibitor of caspase-3 (Ki <1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC50 = 0.2 nM). The N-terminal sequence (residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide.
    FormLyophilized solid
    Chemical formulaC₉₄H₁₅₈N₂₀O₂₇
    Peptide SequenceAc-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO
    Purity≥97% by HPLC
    SolubilityDMSO (5 mg/ml)
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
    Toxicity Standard Handling
    ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
    Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
    Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
    Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
    Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.
    Nicholson, D.W., et al. 1995. Nature 376, 37.
    Tewari, M., et al. 1995. Cell 81, 801.
    Lazebnik, Y.A., et al. 1994. Nature 371, 346.
    Citation
  • Jurg Grunenfelder, et al. (2001) Upregulation of Bcl-2 through caspase-3 inhibition ameliorates ischemia/reperfusion injury in rat cardiac allografts. Circulation 104, I202-I206.