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218769 Caspase-8, Human, Recombinant, E. coli

218769
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
218769-3000U Ampolla de plást. 3000 u
Description
OverviewRecombinant, human caspase-8 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.
M.W. 11,000 and 18,000 (heterodimer).
Catalogue Number218769
Brand Family Calbiochem®
SynonymsFLICE, MACH, Mch5
References
ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Boldin, M.P., et al. 1996. Cell 85, 803.
Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
Muzio, M., et al. 1996. Cell 85, 817.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=368057">Cat. No. 368057</a>) per min at 30°C, pH 7.4.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Applications
Biological Information
Purity≥90% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Caspase-8, Human, Recombinant, E. coli Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Caspase-8, Human, Recombinant, E. coli Certificados de análisis

CargoNúmero de lote
218769

Referencias bibliográficas

Visión general referencias
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Boldin, M.P., et al. 1996. Cell 85, 803.
Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
Muzio, M., et al. 1996. Cell 85, 817.

Folleto

Cargo
Caspases and other Apoptosis Related Tools Brochure

Citas

Título
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
  • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
  • Ficha técnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision06-September-2007 RFH
    SynonymsFLICE, MACH, Mch5
    DescriptionRecombinant, human caspase-8 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.
    FormLiquid
    FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
    Purity≥90% by SDS-PAGE
    Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-pNA (Cat. No. 368057) per min at 30°C, pH 7.4.
    Storage ≤ -70°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
    Toxicity Standard Handling
    ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
    Boldin, M.P., et al. 1996. Cell 85, 803.
    Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
    Muzio, M., et al. 1996. Cell 85, 817.
    Citation
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
  • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.