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219360 Cathepsin D/E Substrate, Fluorogenic - Calbiochem

219360
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
219360-1MG Ampolla de plást. 1 mg
Description
OverviewAn internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.
Catalogue Number219360
Brand Family Calbiochem®
SynonymsMOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
References
ReferencesYasuda, Y., et al. 1999. J. Biochem. 126, 260.
Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.
Product Information
ATP CompetitiveN
FormYellow lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₈₅H₁₂₂N₂₂O₁₉
Chemical formulaC₈₅H₁₂₂N₂₂O₁₉
Hygroscopic Hygroscopic
ReversibleN
Applications
ApplicationCathepsin D/E Substrate, Fluorogenic, is an internally quenched fluorogenic 11-mer peptide that acts as a selective substrate for cathepsins D and E.
Biological Information
Primary TargetSubstrate for cathepsins D and E
Primary Target IC<sub>50</sub>kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively
Purity≥97% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceMOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂
MOCAc = (7-Methoxycoumarin-4-yl)acetyl
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Cathepsin D/E Substrate, Fluorogenic - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Cathepsin D/E Substrate, Fluorogenic - Calbiochem Certificados de análisis

CargoNúmero de lote
219360

Referencias bibliográficas

Visión general referencias
Yasuda, Y., et al. 1999. J. Biochem. 126, 260.
Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-June-2008 RFH
SynonymsMOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
DescriptionAn internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.
FormYellow lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Recommended reaction conditions80 µl of 50 mM sodium acetate buffer, pH 4.0, 10 µl of 200 µM substrate solution, 10 µl of sample, incubate at 40°C for 10 min; terminate reaction with 2 ml of 5% TCA.
Chemical formulaC₈₅H₁₂₂N₂₂O₁₉
Peptide SequenceMOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂
MOCAc = (7-Methoxycoumarin-4-yl)acetyl
Purity≥97% by HPLC
SolubilityDMSO (5 mg/ml)
Storage +2°C to +8°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesYasuda, Y., et al. 1999. J. Biochem. 126, 260.
Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.