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234153 Collagenase, Type I, Clostridium histolyticum

234153
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
234153-1GM Ampolla de plást. 1 gm
Description
Overview

This product has been discontinued.



Crude preparation that contains several collagenases, a sulfhydryl protease, clostripain, and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies.

Note: 1 ABC unit = 1.1 BTC unit.
Catalogue Number234153
Brand Family Calbiochem®
References
ReferencesHarris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med. 291, 557.
Seifter, S., and Harper, E. 1970. Methods Enzymol. 19, 613.
Product Information
CAS number9001-12-1
Activity≥125 ABC units/mg solid
Unit of DefinitionOne ABC unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent per min from collagen at 37°C, pH 7.2. Note: 1 ABC unit = 1.1 BTC (bovine tendon collagen) unit.
EC number3.4.24.3
FormLyophilized
Hygroscopic Hygroscopic
Applications
Biological Information
Physicochemical Information
ContaminantsCaseinase activity: ≤100 FFC unit/500 ABC unit
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38-42/43

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-24-37

Do not breathe dust.
Avoid contact with skin.
Wear suitable gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Collagenase, Type I, Clostridium histolyticum Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Collagenase, Type I, Clostridium histolyticum Certificados de análisis

CargoNúmero de lote
234153

Referencias bibliográficas

Visión general referencias
Harris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med. 291, 557.
Seifter, S., and Harper, E. 1970. Methods Enzymol. 19, 613.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-February-2017 JSW
DescriptionCrude enzyme preparation that contains several collagenases, a sulfhydryl protease, clostripain and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies. Most active on fat cells, adrenal and liver tissue, with an optimal pH of 7.0-8.0.
FormLyophilized
CAS number9001-12-1
EC number3.4.24.3
ContaminantsCaseinase activity: ≤100 FFC unit/500 ABC unit
Activity≥125 ABC units/mg solid
Unit definitionOne ABC unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent per min from collagen at 37°C, pH 7.2. Note: 1 ABC unit = 1.1 BTC (bovine tendon collagen) unit.
SolubilityAqueous buffers (1 mg/ml)
Storage +2°C to +8°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
Toxicity Harmful
Merck USA index14, 2481
ReferencesHarris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med. 291, 557.
Seifter, S., and Harper, E. 1970. Methods Enzymol. 19, 613.