Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

204888 Complement C5, Human

204888
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
204888-250UG Ampolla de plást. 250 μg
Description
OverviewNative, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120 kDa (α) and 75 kDa (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in the assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at peptide bond 74 resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form lytic C5b-9 complement membrane attack complex (MAC).
Catalogue Number204888
Brand Family Calbiochem®
References
ReferencesJanatova, J. 1988. Methods Enzymol. 162, 579.
Wetsel, R.A., et al. 1980. J. Immunol. Methods 35, 319.
Product Information
CAS number80295-53-0
FormLiquid
FormulationIn PBS, pH 7.2.
Applications
Biological Information
Biological activity≥100,000 C₅H₅0 units/mg; ≥60% of the C5 activity in normal human serum on a mg/mg basis
Purity≥95% by SDS-PAGE
SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsIgG, IgA, IgM, albumin, ceruloplasmin or functionally C3, C6, C7, C8, or C9: ≤trace amounts
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Complement C5, Human Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Complement C5, Human Certificados de análisis

CargoNúmero de lote
204888

Referencias bibliográficas

Visión general referencias
Janatova, J. 1988. Methods Enzymol. 162, 579.
Wetsel, R.A., et al. 1980. J. Immunol. Methods 35, 319.

Citas

Título
  • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.
  • Ficha técnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision27-May-2008 RFH
    DescriptionNative, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120,000 (α) and 75,000 (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at the peptide bond 74, resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form the lytic C5b-9 complement membrane attack complex (MAC).
    FormLiquid
    FormulationIn PBS, pH 7.2.
    Concentration Label Please refer to vial label for lot-specific concentration
    SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
    CAS number80295-53-0
    Purity≥95% by SDS-PAGE
    ContaminantsIgG, IgA, IgM, albumin, ceruloplasmin or functionally C3, C6, C7, C8, or C9: ≤trace amounts
    Biological activity≥100,000 C₅H₅0 units/mg; ≥60% of the C5 activity in normal human serum on a mg/mg basis
    Storage ≤ -70°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling
    ReferencesJanatova, J. 1988. Methods Enzymol. 162, 579.
    Wetsel, R.A., et al. 1980. J. Immunol. Methods 35, 319.
    Citation
  • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.