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234409 Complement C9-Depleted Serum, Human

234409
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
234409-1ML Ampolla de plást. 1 ml
Description
OverviewHuman serum depleted of complement component C9 by affinity chromatography.
Catalogue Number234409
Brand Family Calbiochem®
References
Product Information
Unit of Definition<b>Classical Pathway Activity:</b> One C9H50 unit measured by classical pathway activation is defined as the amount of C9 required to yield 50% lysis of 3 x 10⁷EA when incubated in the presence of the recommended volume of C9-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. <b>CH50 Functional Activity upon Reconstitution:</b> One CH50 unit is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. <b>Alternative Pathway Activity:</b> One unit of whole alternative pathway activity (APH50) is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 µl GVB° containing a final Mg-EGTA concentration of 13.3 mM.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM sodium phosphate, pH 7.2 ± 0.2.
PreservativeNone
Applications
Biological Information
SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Complement C9-Depleted Serum, Human Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Complement C9-Depleted Serum, Human Certificados de análisis

CargoNúmero de lote
234409

Citas

Título
  • Jose Yuste, et al. (2006) Roles of the Alternative Complement Pathway and C1q during Innate Immunity to Streptococcus pyogenes. Journal of Immunology 176, 6112-6120.
  • Ficha técnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision09-June-2008 RFH
    DescriptionHuman serum depleted of complement component C9 by affinity chromatography. C9-depleted serum, sterile-filtered through a 0.22 µm filter. No C9 detected by Ouchterlony using various dilutions of goat anti-human C9 antibody.
    FormLiquid
    FormulationIn 150 mM NaCl, 50 mM sodium phosphate, pH 7.2 ± 0.2.
    Concentration Label Please refer to vial label for lot-specific concentration
    Recommended reaction conditionsClassical Pathway Activity Recommended volume C9-Dpl serum per assay: ≤25 µl C9H50 units/mg purified C9 at the recommended input of C9-Dpl: ≥100,000 C9H50 units/mg Functional Activity Ratio: ≥0.60 (C9H50/mg C9 : C9H50/mg C9 in NHS Std.) CH50 Functional Activity upon Reconstitution Reconstitution Ratio: ≥0.60 (CH50/ml C9-Dpl + C9 : CH50/ml NHS Std) Alternative Pathway Activity Input of NHS Standard to yield 1 APH50: ≤20 µl
    SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
    Unit definitionClassical Pathway Activity: One C9H50 unit measured by classical pathway activation is defined as the amount of C9 required to yield 50% lysis of 3 x 10⁷EA when incubated in the presence of the recommended volume of C9-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 µl GVB° containing a final Mg-EGTA concentration of 13.3 mM.
    PreservativeNone
    Storage ≤ -70°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling
    Citation
  • Jose Yuste, et al. (2006) Roles of the Alternative Complement Pathway and C1q during Innate Immunity to Streptococcus pyogenes. Journal of Immunology 176, 6112-6120.