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371206 Dkk-1, His•Tag®, Mouse, Recombinant

371206
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Purity
≥95% by SDS-PAGE

Products

Número de referenciaEmbalaje Cant./Env.
371206-10UG Frasco de vidrio 10 μg
Description
OverviewRecombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
Catalogue Number371206
Brand Family Calbiochem®
References
ReferencesPinzone, J.J. et al. 2009. Blood 113, 517.
Li, J. et al. 2006. Bone 36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C. 2006. Oncogene 25, 7469.
Verani, R. et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M. et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Glinka, A. et al. 1998. Nature 391, 357.
Product Information
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Applications
Biological Information
Biological activityND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Regulatory Review
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Dkk-1, His•Tag®, Mouse, Recombinant Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Dkk-1, His•Tag®, Mouse, Recombinant Certificados de análisis

CargoNúmero de lote
371206

Referencias bibliográficas

Visión general referencias
Pinzone, J.J. et al. 2009. Blood 113, 517.
Li, J. et al. 2006. Bone 36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C. 2006. Oncogene 25, 7469.
Verani, R. et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M. et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Glinka, A. et al. 1998. Nature 391, 357.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-June-2011 RFH
DescriptionRecombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Biological activityND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
SolubilityReconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Regulatory Review
ReferencesPinzone, J.J. et al. 2009. Blood 113, 517.
Li, J. et al. 2006. Bone 36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C. 2006. Oncogene 25, 7469.
Verani, R. et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M. et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Glinka, A. et al. 1998. Nature 391, 357.