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324681 Elastase, Human Neutrophil

324681
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
324681-100UG Frasco de vidrio 100 μg
324681-50UG Ampolla de plást. 50 μg
Description
OverviewNative elastase from human neutrophil. A serine protease that degrades elastin, collagen, and proteoglycans. Its overexpression has been implicated in the development of pulmonary emphysema, rheumatoid arthritis, and cystic fibrosis.
Catalogue Number324681
Brand Family Calbiochem®
SynonymsPMN Elastase
References
ReferencesSiedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855.
Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
Selak, M.A. 1992. Thromb. Haemost. 68, 570.
Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.
Product Information
CAS number9004-06-2
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=454454">Cat. No. 454454</a>) per min at 25°C, pH 8.0.
EC number3.4.21.37
FormLyophilized solid
FormulationSalt-free.
Applications
Biological Information
PurityMajor band by SDS-PAGE
SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity≥20 units/mg protein.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSJX7069000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Elastase, Human Neutrophil Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Elastase, Human Neutrophil Certificados de análisis

CargoNúmero de lote
324681

Referencias bibliográficas

Visión general referencias
Siedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855.
Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
Selak, M.A. 1992. Thromb. Haemost. 68, 570.
Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.

Citas

Título
  • Tianbo Li and Hans G. Folkesson. (2005) RNA interference for αENaC inhibits rat lung fluid absorption in vivo. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L649-L660.
  • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
  • Valerie A. Fadok, et al. (2001) Differential Effects of Apoptotic Versus Lysed Cells on Macrophage Production of Cytokines: Role of Proteases. Journal of Immunology 166, 6847-6854.
  • Ficha técnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-May-2008 RFH
    SynonymsPMN Elastase
    DescriptionNative elastase from human neutrophil. Degrades collagen, elastin, and proteoglycans. Elastase has been implicated in the development of pulmonary emphysema and rheumatoid arthritis.
    FormLyophilized solid
    FormulationSalt-free.
    Recommended reaction conditions
    Human Neutrophil Elastase Protocol 1. Dissolve enzyme in reaction buffer: 100 mM Tris-HCl, pH 7.5, 500 mM NaCl. 2. Prepare the substrate stock solution by dissolving Elastase Substrate I (MeOSuc-Ala-Ala-Pro-Val-pNA, Cat. No. 324696) at 50 mM in DMSO. 3. Just prior to assay, dilute the substrate stock solution with an equal volume of 200 mM Tris-HCl, pH 8.0. Mix well. 4. Prepare the assay mixture by combining 960 µl of reaction buffer with 40 µl of diluted substrate stock solution. 5. Add enzyme to assay mixture, mix, and incubate at 25°C. 6. Monitor the change in absorbance at 410 nm. Use an ε of 8800 at 410 nm to calculate the hydrolysis of nitroanilide.
    SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
    CAS number9004-06-2
    RTECSJX7069000
    EC number3.4.21.37
    PurityMajor band by SDS-PAGE
    Specific activity≥20 units/mg protein.
    Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-pNA (Cat. No. 454454) per min at 25°C, pH 8.0.
    SolubilityReconstitute in 50 mM sodium acetate, pH 5.5, with 200 mM NaCl or in other aqueous buffers. Material is most active near a neutral pH, but to maintain stability upon reconstitution, use in a slightly acidic buffer (pH 5.5) to avoid autolysis.
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.
    Toxicity Standard Handling
    Merck USA index14, 3535
    ReferencesSiedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855.
    Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
    Selak, M.A. 1992. Thromb. Haemost. 68, 570.
    Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.
    Citation
  • Tianbo Li and Hans G. Folkesson. (2005) RNA interference for αENaC inhibits rat lung fluid absorption in vivo. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L649-L660.
  • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
  • Valerie A. Fadok, et al. (2001) Differential Effects of Apoptotic Versus Lysed Cells on Macrophage Production of Cytokines: Role of Proteases. Journal of Immunology 166, 6847-6854.