Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

324691 Elastatinal - CAS 51798-45-9 - Calbiochem

324691
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Empirical FormulaCAS #
C₂₁H₃₆N₈O₇ 51798-45-9

Products

Número de referenciaEmbalaje Cant./Env.
324691-5MG Ampolla de plást. 5 mg
Description
OverviewAn inhibitor of elastase-like serine protease produced by actinomycetes. Inhibits PMSF-stimulated degradation of histone H1 and HMG proteins. Also inhibits α2-macroglobulin-protease complexes. Inhibitory activity: ~0.5 µg/ml using Suc-(Ala)3-pNA as a substrate at 25°C, pH 8.0.
Catalogue Number324691
Brand Family Calbiochem®
References
ReferencesMenendez-Arias, L. 1992. J. Biol. Chem. 267, 11392.
Umezawa, H. 1976. Methods Enzymol. 55, 678.
Product Information
CAS number51798-45-9
ATP CompetitiveN
FormYellow solid
Hill FormulaC₂₁H₃₆N₈O₇
Chemical formulaC₂₁H₃₆N₈O₇
ReversibleN
Applications
Biological Information
Primary TargetElastase-like serine protease produced by actinomycetes
Primary Target IC<sub>50</sub>~0.5 µg/ml using Suc-(Ala)3-pNA as a substrate at 25°C, pH 8.0.
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSLZ9600000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Elastatinal - CAS 51798-45-9 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Elastatinal - CAS 51798-45-9 - Calbiochem Certificados de análisis

CargoNúmero de lote
324691

Referencias bibliográficas

Visión general referencias
Menendez-Arias, L. 1992. J. Biol. Chem. 267, 11392.
Umezawa, H. 1976. Methods Enzymol. 55, 678.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-June-2008 RFH
DescriptionElastase-like serine protease inhibitor produced by actinomycetes. Inhibits PMSF-stimulated degradation of histone H1 and HMG proteins. Also inhibits α2-macroglobulin-protease complexes. Effective at concentrations of 10-100 µM.
FormYellow solid
Recommended reaction conditions
Assay Procedure 1. Dissolve enzyme in reaction buffer: 100 mM Tris-HCl, pH 7.5, 500 mM NaCl. 2. Prepare the substrate stock solution by dissolving Elastase Substrate I (MeOSuc-Ala-Ala-Pro-Val- pNA, Cat. No. 324696) at 50 mM in DMSO. 3. Just prior to assay, dilute the substrate stock solution with an equal volume of 200 mM Tris-HCl, pH 8.0. Mix well. 4. Prepare the assay mixture by combining 960 µl of reaction buffer with 40 µl of diluted substrate stock solution. 5. Add enzyme to assay mixture, mix, and incubate at 25°C. 6. Monitor the change in absorbance at 410 nm. Use an e of 8800 at 410 nm to calculate the hydrolysis of nitroanilide.
CAS number51798-45-9
RTECSLZ9600000
Chemical formulaC₂₁H₃₆N₈O₇
SolubilityH₂O (5 mg/ml)
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesMenendez-Arias, L. 1992. J. Biol. Chem. 267, 11392.
Umezawa, H. 1976. Methods Enzymol. 55, 678.