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324715 Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

324715
Purchase on Sigma-Aldrich

Descripción

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Número de referenciaEmbalaje Cant./Env.
324715-5UG Ampolla de plást. 5 μg
Description
OverviewNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Catalogue Number324715
Brand Family Calbiochem®
References
ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
Product Information
CAS number72561-05-8
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-<i>p</i>NA per min at 25°C, pH 7.7.
EC number3.4.21.50
FormLyophilized
FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
Hygroscopic Hygroscopic
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥200 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes Certificados de análisis

CargoNúmero de lote
324715

Referencias bibliográficas

Visión general referencias
Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-February-2012 RFH
DescriptionNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
FormLyophilized
FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
Recommended reaction conditions
Protocol 1. Dissolve the proteins to be sequenced in 25 mM Tris-HCl, pH 8.5, and 1 mM EDTA. For proteins that are difficult to solubilize, add urea, SDS, or guanidine-HCl to the digestion buffer prior to solubilization. If urea is used, add 20 mmol/l of methylamine. 2. The suggested working concentration is 1:20-1:100 (protease:protein by weight). 3. Incubate for 2-18 h at 37°C.
CAS number72561-05-8
EC number3.4.21.50
Purity≥90% by SDS-PAGE
Specific activity≥200 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.
SolubilityReconstitute in 50 µl of distilled H₂O.
Storage +2°C to +8°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Toxicity Harmful
ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

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Categorías

Life Science Research > Proteins and Enzymes > Other Enzymes