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329871 Erythropoietin, Human, Recombinant, CHO Cells

329871
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
329871-50UG Ampolla de plást. 50 μg
Description
OverviewRecombinant, human erythropoietin (EPO) expressed in CHO cells. EPO is a glycoprotein produced by the kidneys and is involved in red blood cell production. It has a predicted molecular mass of ~21 kDa but migrates at ~37 kDa on SDS-PAGE.
Sialic acid content: > 10 moles/mole of protein.
Catalogue Number329871
Brand Family Calbiochem®
SynonymsEPO, Erythropoietin-α
References
ReferencesFrank, S.J. 2002. Endocrinology 143, 2.
Cerami, A. 2001. Semin. Hematol. 38, 33.
Donnelly, S. 2001. Am. J. Kidney Dis. 38, 415.
Goodnough, L.T. 2001. Curr. Opin. Hematol. 8, 405.
Jelkmann, W, and Hellwig-Burgel, T. 2001. Adv. Exp. Med. Biol. 502, 169.
Siren, A.L., and Ehrenreich, H. 2001. Eur. Arch. Psychiatry Clin. Neurosci 251, 179.
Hansson, G.K., et al. 1992. Scand. J. Clin. Lab. Invest. 52, 35.
Lee, L.G., et al. 1986. Cytometry 7, 35.
Product Information
CAS number11096-26-7
FormLiquid
FormulationIn 300 mM NaCl, 20 mM Na/K phosphate, pH 7.5.
Applications
Biological Information
Purity≥98% by HPLC
Specific Activity≥1.0 X 10⁵ IU/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsEndotoxin: <10 EU/10 µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSKF5885000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C, up to 3 months at -20°C, or up to 6 months at -70°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Erythropoietin, Human, Recombinant, CHO Cells Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Erythropoietin, Human, Recombinant, CHO Cells Certificados de análisis

CargoNúmero de lote
329871

Referencias bibliográficas

Visión general referencias
Frank, S.J. 2002. Endocrinology 143, 2.
Cerami, A. 2001. Semin. Hematol. 38, 33.
Donnelly, S. 2001. Am. J. Kidney Dis. 38, 415.
Goodnough, L.T. 2001. Curr. Opin. Hematol. 8, 405.
Jelkmann, W, and Hellwig-Burgel, T. 2001. Adv. Exp. Med. Biol. 502, 169.
Siren, A.L., and Ehrenreich, H. 2001. Eur. Arch. Psychiatry Clin. Neurosci 251, 179.
Hansson, G.K., et al. 1992. Scand. J. Clin. Lab. Invest. 52, 35.
Lee, L.G., et al. 1986. Cytometry 7, 35.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-November-2007 JSW
SynonymsEPO, Erythropoietin-α
DescriptionRecombinant, human erythropoietin (EPO) expressed in CHO cells. EPO is a glycoprotein hormone produced by the kidneys that is involved in the survival, growth, and differentiation of erythroid precursor cells. Stimulation of erythropoiesis involves the binding of EPO to the extracellular domain of the EPO receptor (EPOR) and triggering of intracellular signaling events, including phosphorylation of the receptor and activation of the JAK-STAT, RAS, and PI3-kinase pathways. Reported to act as a neuroprotective agent. Expressed in and purified from CHO cells. The predicted molecular weight is ~21 kDa, but it migrates at ~37 kDa on SDS-PAGE. Sialic acid content: > 10 moles/mole of protein. Sialic acid content: > 10 moles/mole of protein.
FormLiquid
FormulationIn 300 mM NaCl, 20 mM Na/K phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
CAS number11096-26-7
RTECSKF5885000
Purity≥98% by HPLC
ContaminantsEndotoxin: <10 EU/10 µg protein
Specific activity≥1.0 X 10⁵ IU/mg protein
SolubilityReconstitute with 500 µl H₂O or aqueous buffer.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C, up to 3 months at -20°C, or up to 6 months at -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
Merck USA index14, 3688
ReferencesFrank, S.J. 2002. Endocrinology 143, 2.
Cerami, A. 2001. Semin. Hematol. 38, 33.
Donnelly, S. 2001. Am. J. Kidney Dis. 38, 415.
Goodnough, L.T. 2001. Curr. Opin. Hematol. 8, 405.
Jelkmann, W, and Hellwig-Burgel, T. 2001. Adv. Exp. Med. Biol. 502, 169.
Siren, A.L., and Ehrenreich, H. 2001. Eur. Arch. Psychiatry Clin. Neurosci 251, 179.
Hansson, G.K., et al. 1992. Scand. J. Clin. Lab. Invest. 52, 35.
Lee, L.G., et al. 1986. Cytometry 7, 35.