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341208 Exoenzyme C3, Clostridium botulinum

341208
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
341208-50UG Ampolla de plást. 50 μg
Description
OverviewCatalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho. Inhibits the progression of the cell cycle from G1 to S phase.
Catalogue Number341208
Brand Family Calbiochem®
References
ReferencesUdagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
Yamamoto, M., et al. 1993. Oncogene 8, 1449.
Popoff, A., et al. 1991. Infect. Immun. 59, 3673.
Morii, N., et al. 1988. J. Biol. Chem. 263, 12420.
Hartree, E.F. 1972. Anal. Biochem. 48, 422.
Weber, K., and Osborn, M. 1969. J. Biol. Chem. 244, 4406.
Product Information
CAS number58319-92-9
FormSolid
FormulationLyophilized from 20 mM Tris, 50 mM NaCl, 0.5% sucrose, 0.1% dextran, pH 7.5
Applications
Biological Information
Purity≥90% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 37

Irritating to respiratory system.
S PhraseS: 36

Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 months at -70°C.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Exoenzyme C3, Clostridium botulinum Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Exoenzyme C3, Clostridium botulinum Certificados de análisis

CargoNúmero de lote
341208

Referencias bibliográficas

Visión general referencias
Udagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
Yamamoto, M., et al. 1993. Oncogene 8, 1449.
Popoff, A., et al. 1991. Infect. Immun. 59, 3673.
Morii, N., et al. 1988. J. Biol. Chem. 263, 12420.
Hartree, E.F. 1972. Anal. Biochem. 48, 422.
Weber, K., and Osborn, M. 1969. J. Biol. Chem. 244, 4406.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-June-2016 RB
DescriptionCatalyzes the hydrolysis of NAD. Can be used as a reagent for the detection of Rho proteins. Predominantly catalyzes the ADP ribosylation of the Asn41 amino acid residue in Rho. Treatment of cultured cells with exoenzyme C3 has been shown to induce ADP-ribosylation of RhoA protein and to reduce cell growth rate and saturation density. Its growth inhibitory effects appear to result from blockage at the G1 to S phase transition, causing the accumulation of cells in the G1 phase.
FormSolid
FormulationLyophilized from 20 mM Tris, 50 mM NaCl, 0.5% sucrose, 0.1% dextran, pH 7.5
CAS number58319-92-9
Purity≥90% by SDS-PAGE
SolubilityReconstitute with sterile distilled H₂O
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 months at -70°C.
Toxicity Irritant
ReferencesUdagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
Yamamoto, M., et al. 1993. Oncogene 8, 1449.
Popoff, A., et al. 1991. Infect. Immun. 59, 3673.
Morii, N., et al. 1988. J. Biol. Chem. 263, 12420.
Hartree, E.F. 1972. Anal. Biochem. 48, 422.
Weber, K., and Osborn, M. 1969. J. Biol. Chem. 244, 4406.