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341590 FGF, Basic, Rat, Recombinant

341590
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Purity
≥95% by SDS-PAGE

Products

Número de referenciaEmbalaje Cant./Env.
341590-25UG Frasco de vidrio 25 μg
Description
OverviewRecombinant, rat fibroblast growth factor (FGF) basic consisting of amino acids 11-154 and expressed in E. coli. FGF basic, a member of the FGF family of mitogenic peptides, is known to bind heparine or cell surface heparin sulfate proteoglycans for binding, dimerization, and activation of tyrosine kinase FGF receptors. Also known to bind polysaccharides, proteins, and lipids with lower affinity.
Catalogue Number341590
Brand Family Calbiochem®
SynonymsHBGF-2
References
ReferencesPresta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
Quarto, N., et al. 2005. Gene 356, 49.
Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun. 335, 1239.
Claus, P., et al. 2003. J. Biol. Chem. 278, 479.
Coulier, F., et al. 1997. J. Mol. Evol. 44, 43.
Fernig, D., et al. 1994. Prog. Growth Factor Res. 5, 353.
Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun. 157, 256.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm filtered MOPS, Na₂SO₄, EDTA, DTT, BSA.
Applications
Biological Information
Biological activityED₅₀ = 0.3-1.8 ng/ml as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

FGF, Basic, Rat, Recombinant Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

FGF, Basic, Rat, Recombinant Certificados de análisis

CargoNúmero de lote
341590

Referencias bibliográficas

Visión general referencias
Presta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
Quarto, N., et al. 2005. Gene 356, 49.
Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun. 335, 1239.
Claus, P., et al. 2003. J. Biol. Chem. 278, 479.
Coulier, F., et al. 1997. J. Mol. Evol. 44, 43.
Fernig, D., et al. 1994. Prog. Growth Factor Res. 5, 353.
Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun. 157, 256.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2011 RFH
SynonymsHBGF-2
DescriptionRecombinant, rat fibroblast growth factor (FGF) basic consisting of amino acids 11-154 and expressed in E. coli. FGF basic, a member of the FGF family of mitogenic peptides, is known to bind heparine or cell surface heparin sulfate proteoglycans for binding, dimerization, and activation of tyrosine kinase FGF receptors. Also known to bind polysaccharides, proteins, and lipids with lower affinity.
FormSolid
FormulationLyophilized from 0.2 µm filtered MOPS, Na₂SO₄, EDTA, DTT, BSA.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityED₅₀ = 0.3-1.8 ng/ml as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.
SolubilityReconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesPresta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
Quarto, N., et al. 2005. Gene 356, 49.
Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun. 335, 1239.
Claus, P., et al. 2003. J. Biol. Chem. 278, 479.
Coulier, F., et al. 1997. J. Mol. Evol. 44, 43.
Fernig, D., et al. 1994. Prog. Growth Factor Res. 5, 353.
Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun. 157, 256.