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343242 FLUO 3/AM - CAS 121714-22-5 - Calbiochem

343242
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Empirical FormulaCAS #
C₅₁H₅₀Cl₂N₂O₂₃ 121714-22-5

Products

Número de referenciaEmbalaje Cant./Env.
343242-1MG Frasco de vidrio 1 mg
Description
OverviewMembrane-permeable version of FLUO 3 (Cat. No. 343244). Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.
Catalogue Number343242
Brand Family Calbiochem®
Synonyms1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2ʹ-amino-5ʹ-methylphenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Pentaacetoxymethyl Ester
References
ReferencesCaputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.
Product Information
CAS number121714-22-5
ATP CompetitiveN
FormReddish orange solid
Hill FormulaC₅₁H₅₀Cl₂N₂O₂₃
Chemical formulaC₅₁H₅₀Cl₂N₂O₂₃
ReversibleN
Structure formula ImageStructure formula Image
Applications
Biological Information
Primary TargetCa2+ indicators
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

FLUO 3/AM - CAS 121714-22-5 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

FLUO 3/AM - CAS 121714-22-5 - Calbiochem Certificados de análisis

CargoNúmero de lote
343242

Referencias bibliográficas

Visión general referencias
Caputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.

Folleto

Cargo
Caspases and other Apoptosis Related Tools Brochure
Fluorescent Calcium Probes Technical Bulletin
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-December-2018 JSW
Synonyms1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2ʹ-amino-5ʹ-methylphenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Pentaacetoxymethyl Ester
DescriptionMembrane-permeable version of FLUO 3 (Cat. No. 343244). Cleavage of FLUO 3/AM by cytosolic esterases traps the indicator in the cell. FLUO 3 is almost nonfluorescent at resting calcium levels, but fluorescence levels increase 40-fold upon calcium binding. Unlike all other Ca2+ indicators, FLUO 3/AM is nonfluorescent until it is hydrolyzed by the cell. Since its excitation maximum is at 506 nm, this compound can be used in conjunction with caged compounds, which are released by UV light. Its relatively high Kd for Ca2+ facilitates the measurement of higher peaks of Ca2+ transients than is possible with FURA 2. FLUO 3 has an excitation maximum of 506 nm and an emission maximum of 526 nm in the presence of high and low calcium concentrations.
FormReddish orange solid
Recommended reaction conditionsThe FLUO 3/AM DMSO stock solution should be diluted to 1-5 µM in a physiological buffer. In some cases, use of the non-ionic detergent PLURIONIC® F-127, PROTEIN GRADE® (Cat. No. 540025) at 0.025-0.125% will assist in the dissolution of the dye in aqueous buffers. Cells are normally incubated with the dye for 20-60 min at 20-37°C.
CAS number121714-22-5
Chemical formulaC₅₁H₅₀Cl₂N₂O₂₃
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMF and DMSO (5 mM)
Storage -20°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesCaputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.