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341494 Ferrostatin-1 - Calbiochem

341494
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
341494-25MG Frasco de vidrio 25 mg
Description
OverviewA cell-permeable 3,4-diaminoethylbenzoate-based antioxidant that effectively prevents Glutamate- (5 mM; 3 h) induced neurotoxicity in cultured rat OHSC (by 56%, 65%, and 71%, respectively, in CA1, CA3, and Dentrate gyrus; [Fer-1] = 2 µM, added 0.5 h prior to Glutamate) and Erastin- (Cat. No. 329600; 50 µM; 24 h) induced HT-1080 death (EC50 = 60 nM) by blocking cytosolic and lipid ROS accumulation (by >80% at 0.5 µM in 10 µM Erastin-treated HT-1080 cells). Fer-1 does not prevent H2O2- (Cat. No. 386790) induced necrosis, STS- (Cat. Nos. 569396 & 569397) induced apoptosis, or Iron-independent oxidative death by Rotenone (Cat. No. 557368).
Catalogue Number341494
Brand Family Calbiochem®
SynonymsEthyl-3-amino-4-(cyclohexylamino)benzoate, Ferrostatin1
References
ReferencesSkouta, R., et al. 2014. J. Am. Chem. Soc. 136, In press.
Dixon, S.J., et al. 2012. Cell 149, 1060.
Product Information
FormTan solid
Hill FormulaC₁₅H₂₂N₂O₂
Chemical formulaC₁₅H₂₂N₂O₂
ReversibleY
Structure formula ImageStructure formula Image
Applications
Biological Information
Primary TargetFree radical scavenger
Purity≥99% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Referencias bibliográficas

Visión general referencias
Skouta, R., et al. 2014. J. Am. Chem. Soc. 136, In press.
Dixon, S.J., et al. 2012. Cell 149, 1060.

Folleto

Cargo
Pathways and Biomarkers of Oxidative Stress
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-April-2013 JSW
SynonymsEthyl-3-amino-4-(cyclohexylamino)benzoate, Ferrostatin1
DescriptionA cell-permeable 3,4-diaminoethylbenzoate-based antioxidant ([DPPH]t=10 min/[DPPH]t=0 = 18.8 µM/50 µM; [Fer-1]t=0 = 50 µM) that effectively prevents Glutamate- (5 mM; 3 h) induced neurotoxicity (by PI staining 24 h after glutamate removal) in cultured rat OHSC (by 56%, 65%, and 71%, respectively, in CA1, CA3, and Dentrate gyrus; [Fer-1] = 2 µM, added 0.5 h prior to Glutamate) and Erastin- (Cat. No. 329600; 50 µM; 24 h) induced HT-1080 death (EC50 = 60 nM) by blocking both cytosolic and lipid ROS accumulation (by >80% in 10 µM Erastin-treated HT-1080 cells; [Fer-1] = 0.5 µM) as assessed by ROS probes H2DCFDA (Cat. No. 287810) and C11-BODIPY, respectively. Fer-1 does not prevent H2O2- (Cat. No. 386790) induced necrosis, STS- (Cat. Nos. 569396 & 569397) induced apoptosis, or Iron-independent oxidative death by Rotenone (Cat. No. 557368).
FormTan solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₅H₂₂N₂O₂
Structure formulaStructure formula
Purity≥99% by HPLC
SolubilityDMSO (100 mg/ml)
Storage -20°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesSkouta, R., et al. 2014. J. Am. Chem. Soc. 136, In press.
Dixon, S.J., et al. 2012. Cell 149, 1060.