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341635 Fibronectin, Human Plasma

341635
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
341635-1MG Ampolla de plást. 1 mg
341635-5MG Ampolla de plást. 5 mg
Description
OverviewNative fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
Catalogue Number341635
Brand Family Calbiochem®
References
ReferencesAkiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol. 59, 1.
Krejci, K., and Fritz, H. 1976. FEBS Lett. 64, 152.


Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem. 283, 2297.


Brown, S., et al. 2002. Nature 418, 200.


Kim, M., FEBS Letters 581 1917.
Product Information
CAS number86088-83-7
FormLiquid
FormulationIn PBS, pH 7.3.
Applications
Biological Information
Puritysingle major band by SDS-PAGE
SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Contaminantsnone detected by IEP with antiserum to fibronectin or normal human serum
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsThaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Fibronectin, Human Plasma Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Fibronectin, Human Plasma Certificados de análisis

CargoNúmero de lote
341635

Referencias bibliográficas

Visión general referencias
Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol. 59, 1.
Krejci, K., and Fritz, H. 1976. FEBS Lett. 64, 152.


Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem. 283, 2297.


Brown, S., et al. 2002. Nature 418, 200.


Kim, M., FEBS Letters 581 1917.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-July-2009 RFH
DescriptionNative fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
FormLiquid
FormulationIn PBS, pH 7.3.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Protocol for Coating Tissue Culture Plates With Fibronectin Note: this protocol is meant to serve as a guideline. Optimization may be required for individual cell types.
Note: This protocol is based on the use of 21 cm2 dishes (Corning 25010, Costar 3060, Falcon 3002, or an equivalent 60 mm dish) and 1 mg fibronectin; 1 mg fibronectin is sufficient for coating 10 dishes at 5 µg/cm2.
1. Thaw the fibronectin by placing the vial in a 37°C water bath and leaving it undisturbed until completely thawed. DO NOT DISTURB THE VIAL AT ANY TIME DURING THE THAWING PROCESS! If the vial is disturbed or removed prior to complete thawing, the fibronectin will form a gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing. 2. After thawing, bring the solution to a final volume of 20 ml with sterile, serum-free culture medium that has been pre-warmed to 37°C. This yields a fibronectin solution of 50 µg/ml. 3. Add 2 ml of this solution to each of 10 tissue culture dishes and swirl gently to completely coat the growth surface. 4. Allow the dishes to remain at room temperature for ~45 min to permit binding of the fibronectin to the surface of the dish. 5. Tilt each dish and remove the fibronectin using a sterile pipet. Do not permit the pipet tip to touch or disturb the growth surface. 6. Add the cell suspension in medium directly to the fibronectin-coated dish.
SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number86088-83-7
Puritysingle major band by SDS-PAGE
Contaminantsnone detected by IEP with antiserum to fibronectin or normal human serum
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsThaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
Merck USA index14, 4075
ReferencesAkiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol. 59, 1.
Krejci, K., and Fritz, H. 1976. FEBS Lett. 64, 152.


Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem. 283, 2297.


Brown, S., et al. 2002. Nature 418, 200.


Kim, M., FEBS Letters 581 1917.