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371770 G-Protein Subunit Antibody Set

371770
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

HostAntibody Type
Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
371770-1ST Frasco de vidrio 1 st
Description
OverviewContains 10 µl of each of the following antibodies: Anti-Giα-1 (Cat. No. 371720), Anti-Giα-1 and Giα-2 (Cat. No. 371723), Anti-Giα-3 and Goα (Cat. No. 371726), Anti-Giα-3 (Cat. No. 371729), Anti-Gsα (Cat. No. 371732), Anti-Gq- and G11α (Cat. No. 371751), and Anti-Gβ-Subunit (Cat. No. 371738). Each antibody in the set is sufficient for one Western blot at 1:1000.
See individual antibody descriptions for more details.
Catalogue Number371770
Brand Family Calbiochem®
References
ReferencesMumby, S., et al. 1988. J. Biol. Chem. 263, 2020.
Yatani, A., et al. 1988. Nature 336, 680.
Mumby, S.M., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 265.
Product Information
FormLiquid
FormulationEach antibody is supplied in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
PreservativeNone
Applications
ApplicationG-Protein Subunit Antibody Set. Contains 10 µl of each of Anti-Giα-1, Anti-Giα-1 and Giα-2, Anti-Giα-3 and Goα, Anti-Giα-3, Anti-Gsα, Anti-Gq- and G11α, and Anti-Gβ-Subunit.
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000, all antibodies)
Application CommentsThe specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes encoding each subunit. For immunoblotting: 10 µg purified plasma membranes from rat adipocytes were loaded into each lane and resolved on a 12% acrylamide mini-gel (10 cm x 10 cm). The proteins were transferred to PVDF membranes on a semi-dry blotter and blocked in low detergent blotto (5% milk protein) for 1 hr. The blots were transferred to designated vessels containing the specific antibody diluted in low detergent blotto at 1:1000. Each blot was incubated on a rocker at room temperature with its specific antibody for 2 h, followed by 4 washes with low detergent blotto. Goat anti-rabbit IgG labeled with 125I (approximately 5µCi/µg IgG) was diluted to 500,000 cpm/ml in low detergent blotto, followed by several washes to remove milk proteins. The blots were exposed to X-ray film with intensifying screens for the indicated times. Antibodies should be titrated for optimal results in individual systems.
Biological Information
HostRabbit
IsotypeIgG
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

G-Protein Subunit Antibody Set Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

G-Protein Subunit Antibody Set Certificados de análisis

CargoNúmero de lote
371770

Referencias bibliográficas

Visión general referencias
Mumby, S., et al. 1988. J. Biol. Chem. 263, 2020.
Yatani, A., et al. 1988. Nature 336, 680.
Mumby, S.M., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 265.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-August-2007 RFH
ApplicationImmunoblotting (1:1000, all antibodies)
DescriptionAntibody set containing 10 µl of each of the following antibodies:

Anti-Giα-1 (Cat. No. 371720)
Anti-Giα-1 and Giα-2 (Cat. No. 371723)
Anti-Giα-3 (Cat. No. 371729)
Anti-Giα-3 and Goα (Cat. No. 371726)
Anti-Gsα (Cat. No. 371732)
Anti-G-protein β-subunit (Cat. No. 371738)
Anti-Gqα and G11α (Cat. No. 371751)

Useful for identifying G-protein subunits.
BackgroundGTP-binding regulatory proteins (G-proteins) are heterotrimeric proteins associated with the cell membrane that functionally couple hormones to their receptor systems within the cell. These proteins link signaling pathways that regulate the synthesis of cyclic AMP, as well as other pathways, including activation of phospholipases and mobilization of intracellular calcium. The original term, inhibitory G-proteins (Gi), refers to a family of G-proteins distinguished by differences in the α-subunit of the heterotrimer. It is believed that the different Gi's serve to couple different hormone receptors to different effector systems. However, some G-proteins may not be discriminating because they appear to interact with more than one type of receptor or effector system.
HostRabbit
IsotypeIgG
FormLiquid
FormulationEach antibody is supplied in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
PreservativeNone
CommentsThe specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes encoding each subunit. For immunoblotting: 10 µg purified plasma membranes from rat adipocytes were loaded into each lane and resolved on a 12% acrylamide mini-gel (10 cm x 10 cm). The proteins were transferred to PVDF membranes on a semi-dry blotter and blocked in low detergent blotto (5% milk protein) for 1 hr. The blots were transferred to designated vessels containing the specific antibody diluted in low detergent blotto at 1:1000. Each blot was incubated on a rocker at room temperature with its specific antibody for 2 h, followed by 4 washes with low detergent blotto. Goat anti-rabbit IgG labeled with 125I (approximately 5µCi/µg IgG) was diluted to 500,000 cpm/ml in low detergent blotto, followed by several washes to remove milk proteins. The blots were exposed to X-ray film with intensifying screens for the indicated times. Antibodies should be titrated for optimal results in individual systems.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesMumby, S., et al. 1988. J. Biol. Chem. 263, 2020.
Yatani, A., et al. 1988. Nature 336, 680.
Mumby, S.M., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 265.