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345731 GDF-5, Mouse, Recombinant

345731
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Purity
≥95% by SDS-PAGE

Products

Número de referenciaEmbalaje Cant./Env.
345731-50UG Frasco de vidrio 50 μg
Description
OverviewRecombinant, mouse growth differentiation factor 5 (GDF-5) consisting of amino acids 376-495, expressed in E. coli with and without an N-terminal methionine. GDF-5, a member of the bone morphogenetic protein family, is involved in regulation of myogenesis, bone morphogenesis, neuron differentiation and survival, and chondrogenesis regulation.
Catalogue Number345731
Brand Family Calbiochem®
SynonymsCartilage-derived morphogenetic protein 1 (CDMP-1)
References
ReferencesSettle, S.H., Jr., et al. 2003. Dev. Biol. 254, 116.
Massague, J., et al. 2000. Genes and Dev. 14, 627.
FrancisWest, P.H., et al. 1999.
Development 126, 1035. Inada, M., et al. 1996. BBRC 222, 317.
Nishitoh, H., et al. 1996. J. Biol. Chem. 271, 21345.
Storm, E.E., et al. 1994. Nature 368, 639.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm filter-sterilized Acetonitrile, TFA, BSA.
Applications
ApplicationGDF-5, Mouse, Recombinant, consists of amino acids 376-495 and is expressed in E. coli with and without an N-terminal methionine. Involved in myogenesis, bone morphogenesis & neuron differentiation.
Biological Information
Biological activityED₅₀ = 1-4 µg/ml as measured by induction of alkaline phosphatase production by ATDC5 mouse chondrogenic cells. (Nakamura, K. et al. 1999. Exp. Cell Res. 250, 351.)
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

GDF-5, Mouse, Recombinant Certificados de análisis

CargoNúmero de lote
345731

Referencias bibliográficas

Visión general referencias
Settle, S.H., Jr., et al. 2003. Dev. Biol. 254, 116.
Massague, J., et al. 2000. Genes and Dev. 14, 627.
FrancisWest, P.H., et al. 1999.
Development 126, 1035. Inada, M., et al. 1996. BBRC 222, 317.
Nishitoh, H., et al. 1996. J. Biol. Chem. 271, 21345.
Storm, E.E., et al. 1994. Nature 368, 639.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-September-2011 RFH
SynonymsCartilage-derived morphogenetic protein 1 (CDMP-1)
DescriptionRecombinant, mouse growth differentiation factor 5 (GDF-5) consisting of amino acids 376-495, expressed in E. coli with and without an N-terminal methionine. GDF-5, a member of the bone morphogenetic protein family, is involved in regulation of myogenesis, bone morphogenesis, neuron differentiation and survival, and chondrogenesis regulation.
FormSolid
FormulationLyophilized from 0.2 µm filter-sterilized Acetonitrile, TFA, BSA.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityED₅₀ = 1-4 µg/ml as measured by induction of alkaline phosphatase production by ATDC5 mouse chondrogenic cells. (Nakamura, K. et al. 1999. Exp. Cell Res. 250, 351.)
SolubilityReconstitute to 150 µg/ml in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesSettle, S.H., Jr., et al. 2003. Dev. Biol. 254, 116.
Massague, J., et al. 2000. Genes and Dev. 14, 627.
FrancisWest, P.H., et al. 1999.
Development 126, 1035. Inada, M., et al. 1996. BBRC 222, 317.
Nishitoh, H., et al. 1996. J. Biol. Chem. 271, 21345.
Storm, E.E., et al. 1994. Nature 368, 639.