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345762 GDF-8/Myostatin, Human, Mouse, Rat, Recombinant

345762
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Purity
≥80% by SDS-PAGE

Products

Número de referenciaEmbalaje Cant./Env.
345762-10UG Frasco de vidrio 10 μg
Description
OverviewRecombinant, human mouse rat growth differentiation factor 8 (GDF-8) consisting of amino acids 268-376 and expressed in NS0-derived murine myeloma cells. GDF-8 is expressed in developing and adult skeletal muscle and is 100% identical to mature human, rat, mouse, and cow at the amino acid sequence level. Useful for the inhibition of preadipocyte differentiation to adipocytes.
Catalogue Number345762
Brand Family Calbiochem®
SynonymsMyostatin
References
ReferencesKim, H.S., et al. 2001. Biochem. Biophys. Res. Commun. 281, 902.
Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 9306.
Sharma, M., et al. 1999. J. Cell Physiol. 180, 1.
McPherron, A.C., et al. 1997. Nature 387, 83.
Storm, E.E., et al. 1994. Nature 368, 639.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm filtered Acetonitrile, TFA, BSA.
Applications
ApplicationGDF-8/Myostatin, Human, Mouse, Rat, Recombinant, consists of amino acids 268-376 and is expressed in NS0-derived murine myeloma cells. Useful for inhibiting preadipocyte differentiation to adipocytes.
Biological Information
Biological activityHemoglobin Expression:
ED₅₀ = 2-10 ng/ml as measured by induction of hemoglobin expression in k562 human chronic myelogenous leukemia cells.

Elongation of Animal Caps:
Optimal Elongation of animal caps from Xenpus blastulae.
Purity≥80% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU per/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

GDF-8/Myostatin, Human, Mouse, Rat, Recombinant Certificados de análisis

CargoNúmero de lote
345762

Referencias bibliográficas

Visión general referencias
Kim, H.S., et al. 2001. Biochem. Biophys. Res. Commun. 281, 902.
Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 9306.
Sharma, M., et al. 1999. J. Cell Physiol. 180, 1.
McPherron, A.C., et al. 1997. Nature 387, 83.
Storm, E.E., et al. 1994. Nature 368, 639.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-September-2011 RFH
SynonymsMyostatin
DescriptionRecombinant, human mouse rat growth differentiation factor 8 (GDF-8) consisting of amino acids 268-376 and expressed in NS0-derived murine myeloma cells. GDF-8 is expressed in developing and adult skeletal muscle and is 100% identical to mature human, rat, mouse, and cow at the amino acid sequence level. Useful for the inhibition of preadipocyte differentiation to adipocytes.
FormSolid
FormulationLyophilized from 0.2 µm filtered Acetonitrile, TFA, BSA.
Purity≥80% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU per/µg protein
Biological activityHemoglobin Expression:
ED₅₀ = 2-10 ng/ml as measured by induction of hemoglobin expression in k562 human chronic myelogenous leukemia cells.

Elongation of Animal Caps:
Optimal Elongation of animal caps from Xenpus blastulae.
SolubilityReconstitute to 100 µg/ml in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesKim, H.S., et al. 2001. Biochem. Biophys. Res. Commun. 281, 902.
Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 9306.
Sharma, M., et al. 1999. J. Cell Physiol. 180, 1.
McPherron, A.C., et al. 1997. Nature 387, 83.
Storm, E.E., et al. 1994. Nature 368, 639.