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374805 Hemocyanin, Keyhole Limpet, Megathura crenulata

374805
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
374805-1GM Ampolla de plást. 1 gm
374805-250MG Ampolla de plást. 250 mg
Description
OverviewStable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.
Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
Catalogue Number374805
Brand Family Calbiochem®
SynonymsKLH
References
ReferencesHayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol. 70, 85.
Product Information
CAS number9013-72-3
FormBlue to blue-gray lyophilized solid
FormulationLyophilized from BES buffer, MgSO₄.
PreservativeNone
Applications
ApplicationHemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
Application CommentsProtein concentration: ≥60%
Copper: ≥0.09%
Copper to Protein ratio: ≥1.5 x 10-3
Biological Information
Purity≥90% by size exclusion chromatography
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsDissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Hemocyanin, Keyhole Limpet, Megathura crenulata Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Hemocyanin, Keyhole Limpet, Megathura crenulata Certificados de análisis

CargoNúmero de lote
374805

Referencias bibliográficas

Visión general referencias
Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol. 70, 85.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-March-2011 JSW
SynonymsKLH
DescriptionHemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
BackgroundKeyhole limpet hemocyanin (KLH), a carrier protein with a molecular weight ranging from 3,000,000-7,500,000, has many available primary amines to facilitate protein conjugation. KLH is used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation.
FormBlue to blue-gray lyophilized solid
FormulationLyophilized from BES buffer, MgSO₄.
CAS number9013-72-3
Purity≥90% by size exclusion chromatography
PreservativeNone
CommentsProtein concentration: ≥60%
Copper: ≥0.09%
Copper to Protein ratio: ≥1.5 x 10-3
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsDissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
Toxicity Standard Handling
Merck USA index14, 4647
ReferencesHayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol. 70, 85.