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373275 Hh Signaling Antagonist XII, HPI-1 - CAS 599150-20-6 - Calbiochem

373275
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Descripción

Replacement Information

Tabla espec. clave

Empirical FormulaCAS #
C₂₇H₂₉NO₆ 599150-20-6

Products

Número de referenciaEmbalaje Cant./Env.
373275-10MG 10 mg
Description
OverviewA cell-permeable oxo-tetrahydroquinolinecarboxylate compound that acts as a downstream Hh (Hedgehog) pathway blocker, effectively inhibiting Gli-dependent transcription activity in both Sufu+/+ NIH 3T3 (shh-LIGHT2) and Sufu-/- fibroblast cultures (IC50 = 1.5 & 3 µM, respectively) in a manner similar to two other Hh downstream blockers, HPI-3 (Cat. No. 373276) and GANT-61 (Cat. No. 373401). However, only HPI-1, but not HPI-3 or GANT-61 (no effect at 30 µM), remains effective against Sufu-/- fibroblasts overexpressing Gli1 or Gli2 (IC50 = 6 & 4 µM, respectively). HPI-1 is also shown to inhibit the ligand-independent Hh pathway activation in SmoM2-expressing primary murine CGNPs (Cerebellar Granule Neuron Precursors) and NIH 3T3 (smoM2-LIGHT) cultures.
Catalogue Number373275
Brand Family Calbiochem®
Synonyms2-Methoxyethyl-4-(3-hydroxyphenyl)-7-(2-methoxyphenyl)-2-methyl-5-oxo-4,6,7,8-tetrahydro-1H-quinoline-3-carboxylate
References
ReferencesHyman, J.M., et al. 2009. Proc. Natl. Acad. Sci. USA 106, 14132.
Product Information
CAS number599150-20-6
FormPale yellow solid
Hill FormulaC₂₇H₂₉NO₆
Chemical formulaC₂₇H₂₉NO₆
Structure formula ImageStructure formula Image
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Hh Signaling Antagonist XII, HPI-1 - CAS 599150-20-6 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Hh Signaling Antagonist XII, HPI-1 - CAS 599150-20-6 - Calbiochem Certificados de análisis

CargoNúmero de lote
373275

Referencias bibliográficas

Visión general referencias
Hyman, J.M., et al. 2009. Proc. Natl. Acad. Sci. USA 106, 14132.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-April-2011 RFH
Synonyms2-Methoxyethyl-4-(3-hydroxyphenyl)-7-(2-methoxyphenyl)-2-methyl-5-oxo-4,6,7,8-tetrahydro-1H-quinoline-3-carboxylate
DescriptionA cell-permeable oxo-tetrahydroquinolinecarboxylate compound that acts as a downstream Hh (Hedgehog) pathway blocker, effectively inhibiting Gli-dependent transcription activity in both Sufu+/+ NIH 3T3 (shh-LIGHT2) and Sufu-/- fibroblast cultures (IC50 = 1.5 & 3 µM, respectively) in a manner similar to two other Hh downstream blockers, HPI-3 (Cat. No. 373276) and GANT-61 (Cat. No. 373401). However, only HPI-1, but not HPI-3 or GANT-61 (no effect at 30 µM), remains effective against Sufu-/- fibroblasts overexpressing Gli1 or Gli2 (IC50 = 6 & 4 µM, respectively). HPI-1 is also shown to inhibit the ligand-independent Hh pathway activation in SmoM2-expressing primary murine CGNPs (Cerebellar Granule Neuron Precursors) and NIH 3T3 (smoM2-LIGHT) cultures.
FormPale yellow solid
Intert gas (Yes/No) Packaged under inert gas
CAS number599150-20-6
Chemical formulaC₂₇H₂₉NO₆
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (100 mg/ml)
Storage -20°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesHyman, J.M., et al. 2009. Proc. Natl. Acad. Sci. USA 106, 14132.