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373278 Hh Signaling Antagonist XII, MRT-83 - Calbiochem

373278
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Empirical Formula
C₃₁H₃₀N₄O₅

Products

Número de referenciaEmbalaje Cant./Env.
373278-5MG Frasco de vidrio 5 mg
Description
OverviewA cell-permeable acylguanidine compound that is shown to block Hh signaling and act as a potent, reversible and high-affinity Smo (Smoothened) antagonist. Displays 20-60 -fold greater potency than Cyclopamine (Cat. No. 239803) and competitively inhibits bodipy-cyclopamine binding to Smo (IC50 = 4.6 and 14 nM in HEK293-hSMO and HEK293-mSMO cells, respectively). Represses ShhN (N-myristoylated Shh) signaling (IC50 = 15 nM for Gli-dependent luciferase activity in Shh-light2 cells) and SAG-(Cat. Nos. 566660 & 566661) induced C3H10T1/2 cell differentiation (IC50 = 10 nM in an alkaline phosphatase activity assay). Inhibits ShhN (3 nM) and SAG (10 nM)-mediated proliferation of rat cerebellar granule cell precursors (IC50 ~ 3 and 6 nM, respectively).
Catalogue Number373278
Brand Family Calbiochem®
SynonymsN-(2-Methyl-5-(3-(3,4,5-trimethoxybenzoyl)guanidino)phenyl)-(1,1ʹ-biphenyl)-4-carboxamide
References
ReferencesRoudaut, H., et al. 2011. Mol. Pharmacol. 79, 453.
Product Information
FormYellowish-white solid
Hill FormulaC₃₁H₃₀N₄O₅
Chemical formulaC₃₁H₃₀N₄O₅
Structure formula ImageStructure formula Image
Applications
Biological Information
Purity≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Hh Signaling Antagonist XII, MRT-83 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Hh Signaling Antagonist XII, MRT-83 - Calbiochem Certificados de análisis

CargoNúmero de lote
373278

Referencias bibliográficas

Visión general referencias
Roudaut, H., et al. 2011. Mol. Pharmacol. 79, 453.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-August-2012 JSW
SynonymsN-(2-Methyl-5-(3-(3,4,5-trimethoxybenzoyl)guanidino)phenyl)-(1,1ʹ-biphenyl)-4-carboxamide
DescriptionA cell-permeable acylguanidine compound that is shown to block Hh signaling and act as a potent, reversible and high-affinity Smo (Smoothened) antagonist. Displays 20-60 -fold greater potency than Cyclopamine (Cat. No. 239803) and competitively inhibits bodipy-cyclopamine binding to Smo (IC50 = 4.6 and 14 nM in HEK293-hSMO and HEK293-mSMO cells, respectively). Represses ShhN (N-myristoylated Shh) signaling (IC50 = 15 nM for Gli-dependent luciferase activity in Shh-light2 cells) and SAG-(Cat. Nos. 566660 & 566661) induced C3H10T1/2 cell differentiation (IC50 = 10 nM in an alkaline phosphatase activity assay). Inhibits ShhN (3 nM) and SAG (10 nM)-mediated proliferation of rat cerebellar granule cell precursors (IC50 ~ 3 and 6 nM, respectively).
FormYellowish-white solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₃₁H₃₀N₄O₅
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (100 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesRoudaut, H., et al. 2011. Mol. Pharmacol. 79, 453.