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382180 Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda

382180
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
382180-50UG Ampolla de plást. 50 μg
Description
OverviewFull length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.
Catalogue Number382180
Brand Family Calbiochem®
SynonymsHDAC6, Human, Recombinant
References
ReferencesPandey, U.B., et al. 2007. Nature 447, 859.
Hubbert, C., et al. 2002. Nature 417, 455.
Product Information
Unit of DefinitionOne unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.
FormLiquid
FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 10% glycerol, pH 8.0.
Applications
ApplicationHistone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda, is a full-length HDAC6 fused to GST at the N-terminus. Useful in the study of HDAC6 regulation and for inhibitor screening.
Biological Information
Purity≥80% by SDS-PAGE
Specific Activity≥50 units/µg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda Certificados de análisis

CargoNúmero de lote
382180

Referencias bibliográficas

Visión general referencias
Pandey, U.B., et al. 2007. Nature 447, 859.
Hubbert, C., et al. 2002. Nature 417, 455.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-August-2008 JSW
SynonymsHDAC6, Human, Recombinant
DescriptionFull length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.
FormLiquid
FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 10% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2 0.1 mg/ml BSA, 30 µM fluorescent HDAC substrate, 5 ng/µl HDAC6; incubate at 30° for 60 min.
Purity≥80% by SDS-PAGE
Specific activity≥50 units/µg protein
Unit definitionOne unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesPandey, U.B., et al. 2007. Nature 447, 859.
Hubbert, C., et al. 2002. Nature 417, 455.