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171261 InSolution™ AMPK Inhibitor, Compound C - CAS 866405-64-3 - Calbiochem

171261
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Empirical FormulaCAS #
C₂₄H₂₅N₅O 866405-64-3

Products

Número de referenciaEmbalaje Cant./Env.
171261-1MG Ampolla de plást. 1 mg
Description
OverviewA cell-permeable pyrrazolopyrimidine compound that inhibits against KDR/VEGFR2, ALK2/BMPR-I, AMPK kinase activity (IC50 = 25.1, 148, and 234.6 nM, respectively), while exhibiting much reduced or little effect toward ALK5/TGFβR-I, ZAPK, Syk, PKCθ, PKA, or JAK3. Shown to block both BMP pathway-dependent dorsoventral development (EC100 = 2.5 µM) and VEGF signaling-dependent intersomitic vessel formation (EC50 = 5 µM) in zebrafish embryo in vivo. Commonly used in combination with AMPK activators AICAR (Cat. No. 123040) and/or Metformin (Cat. No. 317240) for studying AMPK-dependent cellular events in vitro and physiological responses in animals in vivo.
Catalogue Number171261
Brand Family Calbiochem®
SynonymsDorsomorphin, BMP Inhibitor I, AMPK Inhibitor I
References
ReferencesHao, J., et al. 2010. ACS Chem.Biol. 5, 245.
Kim, E.K., et al. 2004. J. Biol. Chem. 279, 19970.
Lee, M., et al. 2003. J. Biol. Chem. 278, 39653.
Inoki, K., et al. 2003. Cell 115, 577.
Fryer, L.G., 2002. FEBS Lett. 531, 189.
Zhou, G., et al. 2001. J. Clin. Invest. 108, 1167.
Product Information
CAS number866405-64-3
ATP CompetitiveY
FormLiquid
FormulationSupplied as a 10 mM (1 mg/250 µl) solution of AMPK Inhibitor, Compound C (Cat. No. 171260) in DMSO.
Hill FormulaC₂₄H₂₅N₅O
Chemical formulaC₂₄H₂₅N₅O
Hygroscopic Hygroscopic
ReversibleY
Structure formula ImageStructure formula Image
Applications
Biological Information
Primary TargetAMPK
Primary Target IC<sub>50</sub>25.1, 148, and 234.6 nM, against KDR/VEGFR2, ALK2/BMPR-I, AMPK kinase activity, respectively
Primary Target K<sub>i</sub>EC100 = 2.5 µM against BMP pathway-dependent dorsoventral development; EC50 = 5 µM against VEGF signaling-dependent intersomitic vessel formation in zebrafish embryo in vivo
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Irritant
Storage +2°C to +8°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C). Once opened, stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

InSolution™ AMPK Inhibitor, Compound C - CAS 866405-64-3 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

InSolution™ AMPK Inhibitor, Compound C - CAS 866405-64-3 - Calbiochem Certificados de análisis

CargoNúmero de lote
171261

Referencias bibliográficas

Visión general referencias
Hao, J., et al. 2010. ACS Chem.Biol. 5, 245.
Kim, E.K., et al. 2004. J. Biol. Chem. 279, 19970.
Lee, M., et al. 2003. J. Biol. Chem. 278, 39653.
Inoki, K., et al. 2003. Cell 115, 577.
Fryer, L.G., 2002. FEBS Lett. 531, 189.
Zhou, G., et al. 2001. J. Clin. Invest. 108, 1167.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-March-2012 JSW
SynonymsDorsomorphin, BMP Inhibitor I, AMPK Inhibitor I
DescriptionA cell-permeable pyrrazolopyrimidine compound that inhibits against KDR/VEGFR2, ALK2/BMPR-I, AMPK kinase activity (IC50 = 25.1, 148, and 234.6 nM, respectively), while exhibiting much reduced or little effect toward ALK5/TGFβR-I, ZAPK, Syk, PKCθ, PKA, or JAK3. Shown to block both BMP pathway-dependent dorsoventral development (EC100 = 2.5 µM) and VEGF signaling-dependent intersomitic vessel formation (EC50 = 5 µM) in zebrafish embryo in vivo. Commonly used in combination with AMPK activators AICAR (Cat. No. 123040) and/or Metformin (Cat. No. 317240) for studying AMPK-dependent cellular events in vitro and physiological responses in animals in vivo.
FormLiquid
FormulationSupplied as a 10 mM (1 mg/250 µl) solution of AMPK Inhibitor, Compound C (Cat. No. 171260) in DMSO.
Intert gas (Yes/No) Packaged under inert gas
CAS number866405-64-3
Chemical formulaC₂₄H₂₅N₅O
Structure formulaStructure formula
Purity≥95% by HPLC
Storage +2°C to +8°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C). Once opened, stock solutions are stable for up to 3 months at -20°C.
Toxicity Irritant
ReferencesHao, J., et al. 2010. ACS Chem.Biol. 5, 245.
Kim, E.K., et al. 2004. J. Biol. Chem. 279, 19970.
Lee, M., et al. 2003. J. Biol. Chem. 278, 39653.
Inoki, K., et al. 2003. Cell 115, 577.
Fryer, L.G., 2002. FEBS Lett. 531, 189.
Zhou, G., et al. 2001. J. Clin. Invest. 108, 1167.