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324865 PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb

324865
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
324865-10T Ampolla de plást. 10 t
Description
OverviewRecognizes the ~170 kDa EGFR protein phosphorylated at Tyr845 in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Catalogue Number324865
Brand Family Calbiochem®
SynonymsAnti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)
Application Data
Detection of human epidermal growth factor receptor, phosphorylated on Tyr845, by immunoblotting. Samples: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr845) Rabbit pAb (Cat. No. 324865) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
References
ReferencesSieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.
Positive controlA431 cells stimulated with EGF
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsThe immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb Certificados de análisis

CargoNúmero de lote
324865

Referencias bibliográficas

Visión general referencias
Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-August-2007 RFH
SynonymsAnti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)
ApplicationImmunoblotting (1:1000)
Application Data
Detection of human epidermal growth factor receptor, phosphorylated on Tyr845, by immunoblotting. Samples: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr845) Rabbit pAb (Cat. No. 324865) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr845.
BackgroundEpidermal Growth Factor (EGF) is responsible for a variety of biological effects including mitogenesis and differentiation. The EGF receptor (EGFR) has three domains: the extracellular, transmembrane, and cytoplasmic domain. Upon the binding of EGF to the extracellular domain, dimerization of the receptor occurs and the cytoplasmic domain displays tyrosine kinase activity. This leads to the autophosphorylation of the ~170 kDa glycoprotein on various residues within the cytoplasmic domain as well as the phosphorylation of intracellular substrates. Tyr845 is an autophosphorylation site within EGFR. Phosphorylation of tyrosine 845 is mediated by non-receptor tyrosine kinase c-Src.
HostRabbit
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR
IsotypeIgG
Specieshuman
Positive controlA431 cells stimulated with EGF
FormLiquid
FormulationIn Dulbecco's PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.
Preservative≤0.1% sodium azide
CommentsThe immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesSieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.