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341292 PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb

341292
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
Ch, Dr, Fg, H, M, R Rb Polyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
341292-10T Ampolla de plást. 10 t
Description
OverviewRecognizes the ~125 kDa FAK protein when phosphorylated at Tyr397. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Catalogue Number341292
Brand Family Calbiochem®
SynonymsAnti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
Application Data
Detection of FAK, phosphorylated on Tyr397, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr397) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
References
ReferencesFornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (no Mg²+ and Ca²+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Positive controlChicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesImmunoblotting (1:1000)
Immunohistochemistry (see comments; heat pre-treatment required)
Application CommentsAlthough the antibody was adsorbed against a phospho-peptide corresponding to Pyk2, some cross-reactivity may still be detected in cases where Pyk2 is overexpressed relative to FAK within the same cell system. This antibody has also been reported to work for immunohistochemistry; heat pre-treatment with citrate buffer, pH 6.0 is required. When diluted 1:1000 there is sufficient antibody for 10 miniblots at 10 ml per blot. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
HostRabbit
IsotypeIgG
Species Reactivity
  • Chicken
  • Drosophila
  • Frog
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb Certificados de análisis

CargoNúmero de lote
341292

Referencias bibliográficas

Visión general referencias
Fornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-November-2008 JSW
SynonymsAnti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
ApplicationImmunoblotting (1:1000)
Immunohistochemistry (see comments; heat pre-treatment required)
Application Data
Detection of FAK, phosphorylated on Tyr397, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr397) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated on Tyr397.
BackgroundDiscovered as a substrate for Src, FAK acts as a key element of integrin signaling. FAK is a non-receptor tyrosine kinase that plays a central role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. The Tyr397 autophosphorylation site binds Src family SH2 and the p85 subunit of PI3-Kinase.
HostRabbit
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
IsotypeIgG
SpeciesDrosophila, chicken, frog, human, mouse, rat
Positive controlChicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
FormLiquid
FormulationIn Dulbecco's PBS (no Mg²+ and Ca²+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Preservative≤0.1% sodium azide
CommentsAlthough the antibody was adsorbed against a phospho-peptide corresponding to Pyk2, some cross-reactivity may still be detected in cases where Pyk2 is overexpressed relative to FAK within the same cell system. This antibody has also been reported to work for immunohistochemistry; heat pre-treatment with citrate buffer, pH 6.0 is required. When diluted 1:1000 there is sufficient antibody for 10 miniblots at 10 ml per blot. Antibody should be titrated for optimal results in individual systems.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesFornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.