2500 ReBlot Plus Kit

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      Replacement Information

      Tabla espec. clave

      Key Applications
      Catalogue Number2500
      Brand Family Chemicon®
      Trade Name
      • ReBlot
      • Chemicon
      DescriptionReBlot Plus Kit
      OverviewWestern blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electrophoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.

      Stripping and re-probing of Western blots offers several advantages:

      1) Conservation of samples that are expensive or available only in limited quantities,

      2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies,

      3) Re-analysis of anomalous results and confirmation with the same or a different antibody,

      4) Correcting errors in incubation with the wrong antibody,

      5) Cost savings in reagents and time by reusing the same blot.

      While antigen and antibody-based immunoaffinity matrices, such as Sepharose™ conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded the application of these methods to Western blotting.

      The MILLIPORE Re-Blot Plus Western Blot Recycling Kit contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins.

      Advantages of the Re-Blot Plus Western Blot Recycling Kit include:

      ·*No pungent-smelling b-mercaptoethanol is contained in the Antibody Stripping Solution.

      ·*Antibody stripping is done at room temperature. No heating of blots is required.

      ·*Blots can be stripped of antibodies in approximately 15 minutes at room temperature.

      ·*Blots may be reused in 25 minutes.
      Materials Required but Not Delivered· Standard Blot or blot strips, on nitrocellulose or PVDF/nylon membrane.

      · Blocking Solutions.

      · Plastic Wrap, such as Saran Wrap, for storage of blots that will not be re-probed immediately.

      · Distilled Water, for reagent dilution.

      · Plastic Trays for incubation of blots or blot strips in stripping, washing and blocking solutions.

      · Positive and Negative Stripping Controls. It is recommended that one new strip (not subjected to stripping solution) be included for comparison purposes.
      Product Information
      • Mild Antibody Stripping Solution (10x) - (1 container, 50 mL).
      • Strong Antibody Stripping Solution (10x) - (1 container, 50 mL).
      Detection methodChemiluminescence
      Key Applications
      • Western Blotting
      Application NotesThe MILLIPORE Re-Blot Plus Western Blot Recycling Kit is effective for removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction site.

      The Re-Blot Plus Western Blot Recycling Kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.

      This product is for research use only; not for diagnostic or in vivo use.
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsKit components should be stored at 4°C upon arrival. Product is stable for 3 to 6 months after receipt. If Antibody Stripping Solution crystallizes upon storage, it may be re-dissolved with gentle warming at 37°C before use.

      Note: To prevent reagent degradation secure the cap tightly upon storage. Avoid extended exposure to air.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information



      ReBlot Plus Western Blot Recycling Visual Guide

      ReBlot Plus Kit Ficha datos de seguridad (MSDS)


      Ficha técnica de seguridad del material (MSDS) 

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Ghrelin and des-acyl ghrelin promote differentiation and fusion of C2C12 skeletal muscle cells.
      Filigheddu, Nicoletta, et al.
      Mol. Biol. Cell, 18: 986-94 (2007)  2007

      Mostrar resumen
      17202410 17202410
      A 10-amino acid domain within human T-cell leukemia virus type 1 and type 2 tax protein sequences is responsible for their divergent subcellular distribution.
      Meertens, Laurent, et al.
      J. Biol. Chem., 279: 43307-20 (2004)  2004

      Mostrar resumen
      15269214 15269214
      Prevention of neuron and oligodendrocyte degeneration by interleukin-6 (IL-6) and IL-6 receptor/IL-6 fusion protein in organotypic hippocampal slices.
      Pizzi, Marina, et al.
      Mol. Cell. Neurosci., 25: 301-11 (2004)  2004

      Mostrar resumen
      15019946 15019946

      Ficha técnica

      ReBlot Plus Kit - Data Sheet

      Manuales del usuario

      Re-Blot Plus Western Blot Recycling Kit