Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

113000 Acridine Orange - CAS 65-61-2 - Calbiochem

113000
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₇H₂₀ClN₃65-61-2

Products

Catalogue NumberPackaging Qty/Pack
113000-1GM Glass bottle 1 gm
Description
OverviewA cell-permeable, cationic fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognosis of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry that can be used to provide information about the in situ content, molecular structure, and conformation of many nucleic acid-containing cell constituents.
Catalogue Number113000
Brand Family Calbiochem®
SynonymsAO, 3,6-bis(Dimethylamino)acridine, HCl
References
ReferencesGonzalez, K., et al. 1995. Curr. Eye Res. 14, 269.
Muro-Cacho, C.A., et al. 1995. J. Immunol. 154, 5555.
Olivier, R. 1995. Methods Enzymol. 251, 270.
Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 9165.
Darzynkiewicz, Z. 1994. Methods Cell. Biol. 41, 401.
Delic, J., et al. 1991. Exp. Cell Res. 194, 147.
El-Naggar, A.K., et al. 1991. Cytometry 12, 330.
Lopez, F., et al. 1991. Cytometry 12, 42.
Gurrieri, S., et al. 1990. Biochemistry 29, 3396.
Hermansen, D.K., et al. 1989. Cytometry 10, 739.
Product Information
CAS number65-61-2
ATP CompetitiveN
FormBurnt-orange solid
Hill FormulaC₁₇H₂₀ClN₃
Chemical formulaC₁₇H₂₀ClN₃
Hygroscopic Hygroscopic
ReversibleN
Structure formula ImageStructure formula Image
Applications
Biological Information
Primary TargetInteracts with DNA and RNA
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSAR7601000
Safety Information
R PhraseR: 20/21/22-40

Harmful by inhalation, in contact with skin and if swallowed.
Limited evidence of a carcinogenic effect.
S PhraseS: 36

Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful & Carcinogenic / Teratogenic
Storage +15°C to +30°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 1 month at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Acridine Orange - CAS 65-61-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Acridine Orange - CAS 65-61-2 - Calbiochem Certificates of Analysis

TitleLot Number
113000

References

Reference overview
Gonzalez, K., et al. 1995. Curr. Eye Res. 14, 269.
Muro-Cacho, C.A., et al. 1995. J. Immunol. 154, 5555.
Olivier, R. 1995. Methods Enzymol. 251, 270.
Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 9165.
Darzynkiewicz, Z. 1994. Methods Cell. Biol. 41, 401.
Delic, J., et al. 1991. Exp. Cell Res. 194, 147.
El-Naggar, A.K., et al. 1991. Cytometry 12, 330.
Lopez, F., et al. 1991. Cytometry 12, 42.
Gurrieri, S., et al. 1990. Biochemistry 29, 3396.
Hermansen, D.K., et al. 1989. Cytometry 10, 739.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-April-2008 RFH
SynonymsAO, 3,6-bis(Dimethylamino)acridine, HCl
DescriptionA cell-permeable fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, this cationic dye is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognostication of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry providing information about the in situ content, molecular structure, conformation, and environment of many nucleic acid-containing cell constituents.
FormBurnt-orange solid
CAS number65-61-2
RTECSAR7601000
Chemical formulaC₁₇H₂₀ClN₃
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMF, Ethanol, or H₂O (1 mg/ml)
Storage +15°C to +30°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 1 month at 4°C.
Toxicity Harmful & Carcinogenic / Teratogenic
ReferencesGonzalez, K., et al. 1995. Curr. Eye Res. 14, 269.
Muro-Cacho, C.A., et al. 1995. J. Immunol. 154, 5555.
Olivier, R. 1995. Methods Enzymol. 251, 270.
Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 9165.
Darzynkiewicz, Z. 1994. Methods Cell. Biol. 41, 401.
Delic, J., et al. 1991. Exp. Cell Res. 194, 147.
El-Naggar, A.K., et al. 1991. Cytometry 12, 330.
Lopez, F., et al. 1991. Cytometry 12, 42.
Gurrieri, S., et al. 1990. Biochemistry 29, 3396.
Hermansen, D.K., et al. 1989. Cytometry 10, 739.