113200 Anti-α-Actin Mouse mAb (1A4)

113200
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Bab, H, RbMMonoclonal Antibody

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      113200-500UL
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      In Stock 
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          Plastic ampoule 500 ul
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          Description
          OverviewRecognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
        • Antibody Target Gene Symbol: ACTA2
        • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
        • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
        • Hu Entrez ID: 59 (Related Antibodies: 113200)
        • Rat Entrez ID: 81633
        • Catalogue Number113200
          Brand Family Calbiochem®
          References
          ReferencesRannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
          Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
          Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
          Negative controlFibroblasts, striated muscle, or myocardium
          Positive controlSmooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Frozen Sections
          Paraffin Sections
          Application NotesFrozen Sections (1:50-1:75)
          Immunoblotting (see comments)
          Paraffin Sections (1:50-1:75)
          Application CommentsDoes not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
          ImmunogenHuman Smooth Muscle
          Clone1A4
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Baboon
          • Human
          • Rabbit
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          113200

          References

          Reference overview
          Rannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
          Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
          Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

          Brochure

          Title
          Antibody Sourcebookル Sixth Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-July-2007 RFH
          ApplicationFrozen Sections (1:50-1:75)
          Immunoblotting (see comments)
          Paraffin Sections (1:50-1:75)
          DescriptionMouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
          BackgroundActin is a cytoskeletal protein. Smooth muscle actin forms smaller, less ordered contractile units than actin from skeletal or cardiac muscle. The activity and state of assembly of smooth muscle actin is controlled by calcium-regulated phosphorylation of one of the myosin light chains.
          HostMouse
          Immunogen speciesHuman Smooth Muscle
          Immunogena synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
          Clone1A4
          IsotypeIgG2a
          Speciesbaboon, human, rabbit
          Positive controlSmooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
          Negative controlFibroblasts, striated muscle, or myocardium
          FormLiquid
          FormulationIn 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsDoes not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesRannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
          Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
          Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.