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5202I | Rabies Polyclonal Negative Control

5202I
1 mL  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsFormatHostDetection Methods
      IFFITCGtFluorescent
      Description
      Catalogue Number5202I
      Brand Family Chemicon®
      Trade Name
      • LIGHT DIAGNOSTICS
      • Chemicon
      DescriptionRabies Polyclonal Negative Control
      References
      Product Information
      Detection methodFluorescent
      FormatFITC
      PresentationPurified antibody in protein-buffered solution. Purified antibody in 0.02M PBS, 0.25M NaCl, pH 7.6 contains 0.1% Sodium Azide as a preservative and 15mg/mL BSA as a stabilizer.
      Applications
      Key Applications
      • Immunofluorescence
      Application NotesDILUTION:

      Optimal dilution or concentration was established using the Polyclonal Rabies Reagent

      (Cat. No. 5199i) as a positive control.

      RECOMMENDED

      PROTOCOL:

      1. Allow the acetone-fixed Positive and Negative Control impression slides to equilibrate to room temperature.

      2. If uncoated slides are used, encircle each impression with an ImmuPen or wax pencil.

      3. Add the diluted conjugate directly onto control and test slides. Care must be taken to avoid cross-contamination of slides during the staining procedure.

      4. Incubate in a humid chamber at 37°C for 30 minutes.

      5. Remove excess antibody from the slides by briefly rinsing with PBS. Next, wash the slides for 10 minutes with PBS. Change the PBS once during this time.

      6. Rinse briefly with deionized water and allow to air dry.

      7. Mount coverslip using mounting fluid containing 10-20% glycerol at pH 8.5. (such as Catalog NO 5096)

      8. Examine the mounted slides using a fluorescence microscope.
      Biological Information
      ImmunogenGoat IgG FITC Conjugate
      Concentration1 mg/mL
      HostGoat
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in the United States.
      Usage Statement
      • For in vitro Diagnostic Use
      • CE Mark
      Storage and Shipping Information
      Storage ConditionsMaintain at 2°C to 8°C in undiluted aliquots. When stored at 2°C to 8°C, reagent is stable up to the expiration date printed on the label.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications