171538 AMPK (α1/β1/γ2), His•Tag®, Human, Recombinant, S. frugiperda

171538
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      Prix & Disponibilité

      RéférenceDisponibilité Conditionnement Qté Prix Quantité
      171538-5UG
      Récupération des données relatives à la disponibilité...
      Disponibilité limitéeDisponibilité limitée
      En stock 
      Interrompu(e)
      Disponible en quantités limitées
      Disponibilité à confirmer
        Pour le restant : Nous vous tiendrons informé
          Pour le restant : Nous vous tiendrons informé
          Nous vous tiendrons informé
          Contacter le Service Clients
          Contact Customer Service

          Ampoule plast. 5 μg
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          Description
          OverviewFull length, recombinant, human AMPK consisting of subunits α1/β1/γ2, each fused at the C-terminus to a His•Tag® sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The α-subunit contains a kinase domain, the β-subunit contains a glycogen binding domain, and the γ-subunit contains the regulatory AMP binding domain. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
          Catalogue Number171538
          Brand Family Calbiochem®
          SynonymsAMP Kinase (α1/β1/γ2)
          Application Data
          The activity of AMPK (α1/β1/γ2) was measured as outlined in the Recommended Reaction Conditions.
          References
          ReferencesKahn, B.B., et al. 2005. Cell Metab. 1, 15.
          Hardie, G.D., et al. 2004. J. Cell Sci. 117, 5479.
          Product Information
          Unit of DefinitionKinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP.
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.
          Applications
          Biological Information
          Purity≥70% by SDS-PAGE
          Specific Activity≥897 nmol/min/mg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          FDS

          Titre

          Fiche de données de sécurité des matériaux (FDS) 

          Certificats d'analyse

          TitreNuméro de lot
          171538

          Références bibliographiques

          Aperçu de la référence bibliographique
          Kahn, B.B., et al. 2005. Cell Metab. 1, 15.
          Hardie, G.D., et al. 2004. J. Cell Sci. 117, 5479.
          Fiche technique

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-September-2008 JSW
          SynonymsAMP Kinase (α1/β1/γ2)
          Application Data
          The activity of AMPK (α1/β1/γ2) was measured as outlined in the Recommended Reaction Conditions.
          DescriptionFull length, recombinant, human AMPK consisting of subunits α1/β1/γ2, each fused at the C-terminus to a His•Tag® sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The α-subunit contains a kinase domain, the β-subunit contains a glycogen binding domain, and the γ-subunit contains the regulatory AMP binding domain. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions
          Kinase assay conditions: Dilute purified AMPK in kinase dilution buffer (5 mM MOPS, pH 7.2, 5 mM MgCl2, 2.5 mM β-glycerophosphate, 1 mM EGTA, 400 µM EDTA, 50 ng/µl BSA, 5% glycerol; add DTT to 25 µM just prior to use) to the desired concentration. Mix 10 µl diluted enzyme, 5 µl SAMStide substrate (HMRSAMSGLHLVKRR) (1 mg/ml stock), and 5 µl 500 µM AMP and incubate for 5 min at 30°C. Add 5 µl ATP mixture [(150 µl of 10 mM ATP, 100 µl of 1 mCi/100µl [32P]ATP, and 5.75 ml kinase assay buffer (25 mM MOPS, pH 7.2, 25 mM MgCL2, 12.5 mM β-glycerophosphate, 5 mM EGTA, 2 mM EDTA with 250 µM DTT just prior to use)] and incubate for 15 min at 30°C. Terminate the reaction by spotting 20 µl of the reaction mixture onto a phosphocellulose P81 paper, dry, and wash several times with 1% phosphoric acid prior to counting in the presence of scintillation fluid.
          Purity≥70% by SDS-PAGE
          Specific activity≥897 nmol/min/mg protein
          Unit definitionKinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesKahn, B.B., et al. 2005. Cell Metab. 1, 15.
          Hardie, G.D., et al. 2004. J. Cell Sci. 117, 5479.