197320 | Bcl-2 Converter, D-NuBCP-9-r8, Cell-permeable - Calbiochem

197320
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Empirical Formula
      C₁₀₆H₁₉₁N₄₉O₂₃

      Prix & Disponibilité

      RéférenceDisponibilité Conditionnement Qté Prix Quantité
      197320-2MG
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      En stock 
      Interrompu(e)
      Disponible en quantités limitées
      Disponibilité à confirmer
        Pour le restant : Nous vous tiendrons informé
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          Ampoule plast. 2 mg
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          Description
          OverviewAn all-D-amino acid version of the Nur77-derived minimum Bcl-2/Bcl-B/Bfl-1-conformation converting sequence is conjugated with a D-Arg octamer to facilitate its cellular delivery. CD binding studies reveal a 1:1 stoichiometric binding and that Bcl-2 unstructured loop region (aa 29-90) as the main D-NuBCP-9 interacting site with possible additional involvement of the BH4 domain. D-NuBCP-9-induced apoptosis is Bcl-2-dependent (100% vs 0% inhibition, respectively, of MEF vs Bcl-2-/- MEF colonies formation by 15 µM D-NuBCP-9) and requires the presence of either Bak or Bax (0% inhibition of Bax-/-Bak-/- MEF colonies formation). D-NuBCP-9-induced apoptosis in HeLa cultures is also demonstrated to be potentiated by 114% and 78%, respectively, upon vector expression of Bcl-B or Bfl-1. When applied to MDA-MB435-xenografted mice on the 10th & 13th day after cancer cell implant, D-NuBCP-9 (620 µg/mouse) reportedly suppressed tumor growth (by 80% on day 55) by inducing cancer cell apoptosis in vivo.
          Catalogue Number197320
          Brand Family Calbiochem®
          Synonyms(D)-Ac-fsrslhsll-G-NH(CH₂)₅-CO-rrrrrrrr-NH₂, D-NuBCP-9
          References
          ReferencesKolluri, S.K., et al. 2008. Cancer Cell 14, 285.
          Product Information
          FormLyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₁₀₆H₁₉₁N₄₉O₂₃
          Chemical formulaC₁₀₆H₁₉₁N₄₉O₂₃
          Hygroscopic Hygroscopic
          Applications
          Biological Information
          Purity≥95% by HPLC
          Physicochemical Information
          Peptide Sequence(D)-Ac-phe-ser-arg-ser-leu-his-ser-leu-leu-Gly-NH-(CH₂)₅-CO-arg-arg-arg-arg-arg-arg-arg-arg-NH₂
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          FDS

          Titre

          Fiche de données de sécurité des matériaux (FDS) 

          Certificats d'analyse

          TitreNuméro de lot
          197320

          Références bibliographiques

          Aperçu de la référence bibliographique
          Kolluri, S.K., et al. 2008. Cancer Cell 14, 285.
          Fiche technique

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-September-2009 RFH
          Synonyms(D)-Ac-fsrslhsll-G-NH(CH₂)₅-CO-rrrrrrrr-NH₂, D-NuBCP-9
          DescriptionAn all-D-amino acid version of the Nur77-derived minimum Bcl-2/Bcl-B/Bfl-1-conformation converting sequence is conjugated with a D-Arg octamer to facilitate its cellular delivery. CD binding studies reveal a 1:1 stoichiometric binding and that Bcl-2 unstructured loop region (aa 29-90) as the main D-NuBCP-9 interacting site with possible additional involvement of the BH4 domain. D-NuBCP-9-induced apoptosis is Bcl-2-dependent (100% vs 0% inhibition, respectively, of MEF vs Bcl-2-/- MEF colonies formation by 15 µM D-NuBCP-9) and requires the presence of either Bak or Bax (0% inhibition of Bax-/-Bak-/- MEF colonies formation). D-NuBCP-9-induced apoptosis in HeLa cultures is also demonstrated to be potentiated by 114% and 78%, respectively, upon vector expression of Bcl-B or Bfl-1. When applied to MDA-MB435-xenografted mice on the 10th & 13th day after cancer cell implant, D-NuBCP-9 (620 µg/mouse) reportedly suppressed tumor growth (by 80% on day 55) by inducing cancer cell apoptosis in vivo.
          FormLyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₁₀₆H₁₉₁N₄₉O₂₃
          Peptide Sequence(D)-Ac-phe-ser-arg-ser-leu-his-ser-leu-leu-Gly-NH-(CH₂)₅-CO-arg-arg-arg-arg-arg-arg-arg-arg-NH₂
          Purity≥95% by HPLC
          SolubilityH₂O (1 mg/ml) or DMSO (5 mg/ml)
          Storage +2°C to +8°C
          Hygroscopic
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesKolluri, S.K., et al. 2008. Cancer Cell 14, 285.