203118 Biotin-NHS, Water-Soluble

203118
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      RéférenceDisponibilité Conditionnement Qté Prix Quantité
      203118-50MG
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          Ampoule plast. 50 mg
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          Description
          OverviewReacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.
          Catalogue Number203118
          Brand Family Calbiochem®
          SynonymsSulfobiotin-NHS, Sulfosuccinimidobiotin
          References
          ReferencesLisanti, M.P., et al. 1989. J. Cell Biol. 109, 2145.
          Product Information
          FormOff-white solid
          Hill FormulaC₁₄H₁₈N₃O₈S₂ · Na
          Chemical formulaC₁₄H₁₈N₃O₈S₂ · Na
          Applications
          Biological Information
          Purityhomogeneous single spot by TLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          FDS

          Titre

          Fiche de données de sécurité des matériaux (FDS) 

          Certificats d'analyse

          TitreNuméro de lot
          203118

          Références bibliographiques

          Aperçu de la référence bibliographique
          Lisanti, M.P., et al. 1989. J. Cell Biol. 109, 2145.

          Brochure

          Titre
          Protein Modifications Tools
          Fiche technique

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-May-2008 RFH
          SynonymsSulfobiotin-NHS, Sulfosuccinimidobiotin
          DescriptionWater-soluble form of biotin-NHS. Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.
          FormOff-white solid
          Recommended reaction conditions
          Buffers containing amines (such as Tris or glycine) should be avoided because they can compete with the reaction. It is also very important that the stock solution be prepared just prior to addition of the reaction mixture, because these compounds are prone to hydrolysis by water. Water-soluble crosslinkers can be dissolved in water or buffer at a concentration of 10-25 mM. Phosphate buffers may precipitate sulfo-N-hydroxysuccinimide (sulfo-NHS) containing compounds. In addition, the solid crosslinker can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions, but can be minimized by using more concentrated protein solution (greater than 5 mg/ml). If you prefer to work with stock solutions, we recommend that you dissolve the crosslinker in anhydrous DMSO at a concentration of 10-25 mM. This solution can then be diluted into the buffer of your choice just prior to use. General Handling 1. Store desiccated at 4°C. 2. Warm contents to room temperature completely before opening. Allow at least 30 min. 3. Wipe the vial to remove any moisture. 4. After opening, store under nitrogen. 5. Protect from moisture.
          Chemical formulaC₁₄H₁₈N₃O₈S₂ · Na
          Purityhomogeneous single spot by TLC
          SolubilitydH₂O (4 mg/ml) or DMSO (10 mg/ml)
          Storage +2°C to +8°C
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
          Toxicity Standard Handling
          ReferencesLisanti, M.P., et al. 1989. J. Cell Biol. 109, 2145.